Anti-Human CXCR1 (extracellular) Antibody | Alomone Labs ACR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CXCR1 (extracellular) Antibody

catalog :

ACR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of CXCR1 in human Jurkat acute T cell leukemia cell lysate: - 1. Anti-Human CXCR1 (extracellular) Antibody (#ACR-011), (1:200).2. Anti-Human CXCR1 (extracellular) Antibody, preincubated with Human CXCR1 (extracellular) Blocking Peptide (#BLP-CR011).

image 2 :

Peptide (C)SNITDPQMW DFDDLN corresponding to amino acid residues 2-16 of human CXCR1 (AccessionP25024). Extracellular N-terminus.

image 3 :

Cell surface detection of CXCR1 in Jurkat living cells: - ___Unstained cells.___Cells +Anti-HumanCXCR1 (extracellular)Antibody (#ACR-011) (10 g/5x105cells).

product information

CAT :

ACR-011

SKU :

ACR-011-CF_0.2 ml

Product Name :

Anti-Human CXCR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25024

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CR011

Homology :

Specific to human CXCR1

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)SNITDPQMW DFDDLN, corresponding to amino acid residues 2-16 of human CXCR1

Product Page - Scientific background :

Chemokine receptors belong to the seven transmembrane domain G-protein coupled receptor superfamily and are differentially expressed in diverse cell types. In leukocytes, chemokines coordinate development, differentiation, anatomic distribution, trafficking, and effector functions and thereby regulate innate and adaptive immune responses.To date, 18 chemokine receptors are known and designated as CXCR1-5, CCR1-11, XCR1, and CX3CR1 based on their specific chemokine preferences.The CXC chemokine receptors specifically bind and respond to cytokines of the CXC chemokine family. CXCR1 and CXCR2 were the first chemokine receptor subtypes to be defined. They are the only known mammalian receptors for ELR+ CXC chemokines [CXC chemokines which contain the tripeptide motif glutamic acid-leucine-arginine (ELR) N-terminal to the first cysteine].CXCR1 and CXCR2 both bind IL-8, with high affinity; they do not bind other types of chemokines. CXCR1 and CXCR2 are the major chemokine receptors expressed on neutrophils. They appear to operate mainly in acute inflammation and innate immunity.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181

Short Description :

A Rabbit Polyclonal Antibody to CXCR1 Chemokine Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human CXCR1. Anti-Human CXCR1 (extracellular) Antibody (#ACR-011) can be used for western blot and indirect flow cytometry applications. It has been designed to recognize human CXCR1.

Negative Control :

BLP-CR011

Positive Control :

Synonyms :

C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CXCR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage