Anti-Calcium Sensing Receptor (extracellular) Antibody | Alomone Labs ACR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Calcium Sensing Receptor (extracellular) Antibody

catalog :

ACR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Jäger E, Murthy S, Schmidt C, Hahn M, Strobel S, Peters A, et al. Calcium-sensing receptor-mediated NLRP3 inflammasome response to calciprotein particles drives inflammation in rheumatoid arthritis. Nat Commun. 2020;11:4243 pubmed publisher
Zhang X, Hong S, Qi S, Liu W, Zhang X, Shi Z, et al. NLRP3 Inflammasome Is Involved in Calcium-Sensing Receptor-Induced Aortic Remodeling in SHRs. Mediators Inflamm. 2019;2019:6847087 pubmed publisher
Graca J, Schepelmann M, Brennan S, Reens J, Chang W, Yan P, et al. Comparative expression of the extracellular calcium-sensing receptor in the mouse, rat, and human kidney. Am J Physiol Renal Physiol. 2016;310:F518-33 pubmed publisher

image

image 1 :

Western blot analysis of Colo205 (lanes 1 and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates: - 1,2,5. Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004), (1:200).3,4,6. Anti-Calcium Sensing Receptor (extracellular) Antibody, preincubated with Calcium Sensing Receptor (extracellular) Blocking Peptide (#BLP-CR004).

image 2 :

Expression of CaSR in rat brain - Immunohistochemical staining of rat hippocampal CA1 region using Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004). A. CaSR staining appears in the soma and appical dendrites of CA1 pyramidal neurons (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merge image of A and B pannels

image 3 :

Cell surface detection of CaSR in live Jurkat (human acute T cell leukemia) cells: - ___Unstained cells.___Cells +Anti-Calcium SensingReceptor (extracellular)Antibody (#ACR-004) (10 g/5x105cells).

product information

CAT :

ACR-004

SKU :

ACR-004-CF_0.2 ml

Product Name :

Anti-Calcium Sensing Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41180

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR004

Homology :

Rat, mouse, bovine, canis - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR

Product Page - Scientific background :

The Calcium-sensing receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism such as parathyroid cells and the kidneys.1,2The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and the pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas, lower levels of expression were found in the cancerous tissue compared to normal colon tissue.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Short Description :

A Rabbit Polyclonal Antibody to Calcium Sensing Receptor

Long Description :

Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry, and live cell imaging applications. It has been designed to recognize CaSR from human, rat, and mouse samples.

Negative Control :

BLP-CR004

Positive Control :

Synonyms :

CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CASR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage