Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody | Alomone Labs ACR-004-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody

catalog :

ACR-004-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Jäger E, Murthy S, Schmidt C, Hahn M, Strobel S, Peters A, et al. Calcium-sensing receptor-mediated NLRP3 inflammasome response to calciprotein particles drives inflammation in rheumatoid arthritis. Nat Commun. 2020;11:4243 pubmed publisher

image

image 1 :

Cell surface detection of CaSR in live intact human THP-1 monocytic leukemia cells: Cell surface detection of CaSR in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody (#ACR-004-F), 2.5 µg.

product information

CAT :

ACR-004-F

SKU :

ACR-004-F_50 mcl

Product Name :

Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41180

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR004

Homology :

Rat, mouse, bovine, dog - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR

Product Page - Scientific background :

The calcium-sensing receptor (CaSR) is a class C G-protein-coupled receptor encoded by a single copy gene. CaSR plays a major role in the maintenance of a physiological serum ionized Ca2+ concentration by regulating the circulating levels of parathyroid hormone1.The N-terminus of the receptor is comprised of a signal peptide of 19 amino acids. This terminus directs the nascent CaSR polypeptide into the lumen of the endoplasmic reticulum (ER) and is then cleaved. The extracellular domain (ECD) of the mature protein has approximately 600 amino acids, comprised of a bi-lobed Venus-flytrap-like domain (VFT) connected by a cysteine-rich region and a short peptide linker to the seven transmembrane domain (TMD) with an intracellular C-terminal tail of 216 amino acids. CaSR functions as a homodimer with both covalent and noncovalent interactions between the VFT domains of each monomer. Ca2+ binds in the cleft between the two lobes of each VFT causing the lobes to close on one another and the VFT to rotate with transfer of a conformational change to the cysteine-rich region.In the parathyroid gland, up-regulation of CaSR by 1,25-dihydroxyvitamin D [1,25(OH)2D] makes the gland more responsive to extracellular Ca2+ and parathyroid hormone (PTH) secretion is reduced. While somatic mutations in CASR are rare, parathyroid glands from patients with primary or severe uremic secondary hyperparathyroidism have reduced CaSR expression2.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Short Description :

A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize CaSR from human, rat, and mouse samples. Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody (#ACR-004-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CASR

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACR-004), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage