Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody | Alomone Labs ACR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody

catalog :

ACR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ceballos Guti xe9 rrez A, Rodr xed guez Hern xe1 ndez A, xc1 lvarez Valadez M, Lim xf3 n Miranda S, Andrade F, Figueroa Guti xe9 rrez A, et al. ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure. Nanomaterials (Basel). 2021;11: pubmed publisher

image

image 1 :

Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysate (lanes 1 and 2) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 4): - 1,3. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (#ACR-003), (1:200).2,4. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody, preincubated with Human Cannabinoid Receptor 2 (extracellular) Blocking Peptide (#BLP-CR003).

image 2 :

Peptide (C)NGSKDGLDSNPMKD corresponding to amino acid residues 11-24 of human CB2 receptor (AccessionP34972). Extracellular N-terminus.

image 3 :

Cell surface detection of CB2 receptor inlive intacthuman HL-60 promyelocytic leukaemiacells: - ___ Cells + goat-anti-rabbit-FITC alone.___Cells + Anti-Human CannabinoidReceptor 2 (extracellular) Antibody (#ACR-003) (5 ?g)+goat-anti-rabbit-FITC.

product information

CAT :

ACR-003

SKU :

ACR-003-CF_0.2 ml

Product Name :

Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34972

Applications :

IC IF IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CR003

Homology :

Canis, bovine - 10/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human CB2 receptor

Product Page - Scientific background :

Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relation between CB2 expression and tumor malignancy was demonstrated.8 Activation of CB2R in vivo by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

CB2, CNR2

Short Description :

A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human CB2 receptor. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (#ACR-003) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize CB2 from human samples. The antibody is not recommended for use on rat and mouse samples.

Negative Control :

BLP-CR003

Positive Control :

Synonyms :

CB2, CNR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNR2

AB Specifictiy :

Not recommended to use with rat and mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage