Anti-Cannabinoid Receptor 2 Antibody | Alomone Labs ACR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Cannabinoid Receptor 2 Antibody

catalog :

ACR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Ceballos Guti xe9 rrez A, Rodr xed guez Hern xe1 ndez A, xc1 lvarez Valadez M, Lim xf3 n Miranda S, Andrade F, Figueroa Guti xe9 rrez A, et al. ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure. Nanomaterials (Basel). 2021;11: pubmed publisher
Salaga M, Binienda A, Piscitelli F, Mokrowiecka A, Cygankiewicz A, Verde R, et al. Systemic administration of serotonin exacerbates abdominal pain and colitis via interaction with the endocannabinoid system. Biochem Pharmacol. 2019;161:37-51 pubmed publisher
Zhang H, Shen H, Jordan C, Liu Q, Gardner E, Bonci A, et al. CB2 receptor antibody signal specificity: correlations with the use of partial CB2-knockout mice and anti-rat CB2 receptor antibodies. Acta Pharmacol Sin. 2019;40:398-409 pubmed publisher
Li M, Suchland K, Ingram S. Compensatory Activation of Cannabinoid CB2 Receptor Inhibition of GABA Release in the Rostral Ventromedial Medulla in Inflammatory Pain. J Neurosci. 2017;37:626-636 pubmed publisher
Zhang H, Gao M, Shen H, Bi G, Yang H, Liu Q, et al. Expression of functional cannabinoid CB2 receptor in VTA dopamine neurons in rats. Addict Biol. 2017;22:752-765 pubmed publisher

image

image 1 :

Western blot analysis of rat RBL basophilic leukemia (lanes 1 and 3), rat C6 brain glioma (lanes 2 and 4) and rat lung (lanes 5 and 6) lysates: - 1,2,5. Anti-Cannabinoid Receptor 2 Antibody (#ACR-002), (1:200).3,4,6. Anti-Cannabinoid Receptor 2 Antibody, preincubated with Cannabinoid Receptor 2 Blocking Peptide (#BLP-CR002).

image 2 :

Expression of CB2 receptor in rat dermis - Immunohistochemical staining of paraffin embedded section of rat dermis using Anti-Cannabinoid Receptor 2 Antibody (#ACR-002), (1:100). CB2 is expressed in sebaceous glands and ducts of sweat glands in the reticular dermis. Hematoxilin is used as the counterstain.

image 3 :

Expression of CB2 receptor in rat dermis - Immunohistochemical staining of paraffin embedded section of rat dermis usingAnti-Cannabinoid Receptor2Antibody (#ACR-002) (1:100). CB2 is expressed in sebaceous glands and ducts of sweat glands in the reticular dermis. Hematoxilin is used as the counterstain.

product information

CAT :

ACR-002

SKU :

ACR-002-CF_0.2 ml

Product Name :

Anti-Cannabinoid Receptor 2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QZN9

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR002

Homology :

Mouse - identical Human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DRQVPGIARMRLDVR, corresponding to amino acid residues 228-242 of rat CB2 receptor

Product Page - Scientific background :

Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 receptor is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in the brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.6 In human astrocytoma a direct relationship between CB2 expression and tumor malignancy was demonstrated. Activation of CB2 in vivo by its agonist JWH-133, completely blocked cell growth. In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in the internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.7

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CB2, CNR2

Short Description :

A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2

Long Description :

Alomone is pleased to offer a new antibody directed against an intracellular epitope of the rat CB2 receptor. Anti-Cannabinoid Receptor 2 Antibody (#ACR-002) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CB2 receptor in rat, mouse and human.

Negative Control :

BLP-CR002

Positive Control :

Synonyms :

CB2, CNR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage