Anti-Cannabinoid Receptor 1 (extracellular) Antibody | Alomone Labs ACR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Cannabinoid Receptor 1 (extracellular) Antibody

catalog :

ACR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Qi M, Kaddis J, Chen K, Rawson J, Omori K, Chen Z, et al. Chronic marijuana usage by human pancreas donors is associated with impaired islet function. PLoS ONE. 2021;16:e0258434 pubmed publisher
Ceballos Guti xe9 rrez A, Rodr xed guez Hern xe1 ndez A, xc1 lvarez Valadez M, Lim xf3 n Miranda S, Andrade F, Figueroa Guti xe9 rrez A, et al. ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure. Nanomaterials (Basel). 2021;11: pubmed publisher
Esteban P, Garcia Ovejero D, Paniagua Torija B, Moreno Luna R, Arredondo L, Zimmer A, et al. Revisiting CB1 cannabinoid receptor detection and the exploration of its interacting partners. J Neurosci Methods. 2020;337:108680 pubmed publisher
Pietrovito L, Iozzo M, Bacci M, Giannoni E, Chiarugi P. Treatment with Cannabinoids as a Promising Approach for Impairing Fibroblast Activation and Prostate Cancer Progression. Int J Mol Sci. 2020;21: pubmed publisher
Udi S, Hinden L, Ahmad M, Drori A, Iyer M, Cinar R, et al. Dual inhibition of cannabinoid CB1 receptor and inducible NOS attenuates obesity-induced chronic kidney disease. Br J Pharmacol. 2020;177:110-127 pubmed publisher
Salaga M, Binienda A, Piscitelli F, Mokrowiecka A, Cygankiewicz A, Verde R, et al. Systemic administration of serotonin exacerbates abdominal pain and colitis via interaction with the endocannabinoid system. Biochem Pharmacol. 2019;161:37-51 pubmed publisher
HÃ¡jkovÃ¡ A, TechlovskÃ¡ Å, DvoÅ™Ã¡kovÃ¡ M, Chambers J, KumpoÅ¡t J, HubÃ¡lkovÃ¡ P, et al. SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner. Neuropharmacology. 2016;107:201-214 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane proteins: - 1. Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:200).2. Anti-Cannabinoid Receptor 1 (extracellular) Antibody, preincubated with Cannabinoid Receptor 1 (extracellular) Blocking Peptide (#BLP-CR001).

image 2 :

Expression of CB1 receptor in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:100). A. CB1 is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1 in GABAergic cells.

image 3 :

Expression of CB1 receptor in mouse hippocampus - Immunohistochemical staining ofmouse hippocampus usingAnti-Cannabinoid Receptor 1 (extracellular)Antibody (#ACR-001) (1:100). A. CB1 is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1 in GABAergic cells.

product information

CAT :

ACR-001

SKU :

ACR-001-CF_0.2 ml

Product Name :

Anti-Cannabinoid Receptor 1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20272

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR001

Homology :

Human - identical; mouse - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor

Product Page - Scientific background :

Cannabinoids have been used in Eastern medicine for many years as pain relievers.1 Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 receptor) and cannabinoid receptor 2 (CB2 receptor).2 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.2 Both receptors belong to the G-protein coupled receptor (GPCR) superfamily. CB1 was shown to modulate several Ca2+ and K+ ion channels.2,3CB1 is primarily expressed in the central nervous system. However, expression of CB1 is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.3,4CB1 is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.5,6 Growth inhibition of tumor cells was demonstrated following mixed CB1/CB2 agonist treatment in both prostate and non-melanoma skin cancers.5,6 Through their interaction with CB1, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1 an attractive therapeutic target for the treatment of obesity and eating disorders.7

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

CB1, CB-R, CNR1, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor

Short Description :

A Rabbit Polyclonal Antibody to Cannabinoid Receptor 1

Long Description :

Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) is a highly specific antibody directed against an epitope of the rat CB1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize CB1 in human, mouse, and rat samples.

Negative Control :

BLP-CR001

Positive Control :

Synonyms :

CB1, CB-R, CNR1, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage