Anti-SERCA3 Antibody | Alomone Labs ACP-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SERCA3 Antibody

catalog :

ACP-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Christodoulou P, Yiallouris A, Michail A, Christodoulou M, Politis P, Patrikios I. Altered SERCA Expression in Breast Cancer. Medicina (Kaunas). 2021;57: pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-SERCA3 Antibody (#ACP-014), (1:200).3,4. Anti-SERCA3 Antibody, preincubated with SERCA3 Blocking Peptide (#BLP-CP014).

image 2 :

Expression of SERCA3 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SERCA3 Antibody (#ACP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SERCA3 immunoreactivity (green) appears in the molecular layer (MOL) and Purkinje cells (arrows) but not in the granule layer (GL) or cerebellum sections. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACP-014

SKU :

ACP-014-CF_0.2 ml

Product Name :

Anti-SERCA3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18596

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP014

Homology :

Mouse - 11/13 amino acid residues identical; human - 10/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DEALKYLSRHHVD, corresponding to amino acid residues 981-993 of rat SERCA3

Product Page - Scientific background :

SERCA3 belongs to the family of P-type ion translocating ATPases and is responsible for maintaining a Ca2+ concentration gradient between the cytosol and the endoplasmic reticulum. SERCA3 transports two Ca2+ ions in the endoplasmic reticulum (ER) per ATP molecule hydrolyzed1-3. SERCA proteins are transmembrane and consist of a single polypeptide chain folded into four major domains. SERCA proteins are encoded by 3 genes, ATP2A1, ATP2A2, and ATP2A3. All three genes produce several alternate transcripts and protein isoforms.SERCA3 protein is mostly expressed in non-muscle cells, especially in endothelial and hematopoietic cells. Minor expression can be detected in muscle cells2,3.SERCA3 knockout mice exhibit prolonged tail bleeding time and rebleeding1. Mutations in the SERCA3 gene are associated with type 2 diabetes. The protein is also implicated in several cardiac disorders2,3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3

Short Description :

A Rabbit Polyclonal Antibody to SERCA3 Pump

Long Description :

Anti-SERCA3 Antibody (#ACP-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SERCA3 from rat, mouse, and human samples.

Negative Control :

BLP-CP014

Positive Control :

Synonyms :

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage