Anti-SERCA2 Antibody | Alomone Labs ACP-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SERCA2 Antibody

catalog :

ACP-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Bächinger D, Egli H, Goosmann M, Monge Naldi A, Eckhard A. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear. Cell Tissue Res. 2019;378:163-173 pubmed publisher
Fan H, Luo T, Zhao W, Mu Y, Yang Y, Guo W, et al. Functional interaction of Junctophilin 2 with small conductance Ca2+ -activated potassium channel subtype 2(SK2) in mouse cardiac myocytes. Acta Physiol (Oxf). 2017;: pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes (lanes 1 and 5), mouse heart membranes (lanes 2 and 6), rat PC12 pheochromocytoma cell line lysate (lanes 3 and 7) and human Jurkat T cell leukemia cell line lysate (lanes 4 and 8): - 1-4. Anti-SERCA2 Antibody (#ACP-012), (1:200).5-8. Anti-SERCA2 Antibody, preincubated with SERCA2 Blocking Peptide (#BLP-CP012).

image 2 :

Peptide CTPNKPSRTSMSK corresponding to amino acid residues 498-510 of rat SERCA2 (AccessionP11507). 2ndcytoplasmic loop.

image 3 :

Expression of SERCA2 in mouse C2C12 cells - Immunocytochemical staining of fixed and permeabilized mouse myoblast C2C12 cells. A. Cells were stained usingAnti-SERCA2Antibody (#ACP-012) (1:300) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merged image of SERCA2 labeling with a live image of the cells. Cell nuclei were visualized using Hoechst 33342 (blue).

product information

CAT :

ACP-012

SKU :

ACP-012-CF_0.2 ml

Product Name :

Anti-SERCA2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11507

Applications :

IC IF WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP012

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd cytoplasmic loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2

Product Page - Scientific background :

Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization3,4.

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2

Short Description :

A Rabbit Polyclonal Antibody to SERCA2 Pump

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat SERCA2. Anti-SERCA2 Antibody (#ACP-012) can be used in western blot and immunocytochemistry applications and was designed to recognize SERCA2 from rat, mouse and human samples.

Negative Control :

BLP-CP012

Positive Control :

Synonyms :

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage