Anti-SERCA1 Antibody | Alomone Labs ACP-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SERCA1 Antibody

catalog :

ACP-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Bächinger D, Egli H, Goosmann M, Monge Naldi A, Eckhard A. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear. Cell Tissue Res. 2019;378:163-173 pubmed publisher
Li L, Li J, Zuo Y, Dang D, Frost J, Yang Q. Activation of KCNQ Channels Prevents Paclitaxel-Induced Peripheral Neuropathy and Associated Neuropathic Pain. J Pain. 2019;20:528-539 pubmed publisher
Rouwette T, Sondermann J, Avenali L, Gomez Varela D, Schmidt M. Standardized Profiling of The Membrane-Enriched Proteome of Mouse Dorsal Root Ganglia (DRG) Provides Novel Insights Into Chronic Pain. Mol Cell Proteomics. 2016;15:2152-68 pubmed publisher

image

image 1 :

Western blot analysis of mouse C2C12 myoblast cell line lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4): - 1,2. Anti-SERCA1 Antibody (#ACP-011), (1:200).3,4. Anti-SERCA1 Antibody, preincubated with SERCA1 Blocking Peptide (#BLP-CP011).

image 2 :

Expression of SERCA1 in rat skeletal muscle - Immunohistochemical staining of rat skeletal muscle paraffin-embedded sections using Anti-SERCA1 Antibody (#ACP-011), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. SERCA1 labeling appears in the muscle fibers, in a pattern that could indicate the location of the sarcoplasmic reticulum. The endomysium, surrounding muscle fibers, is not stained. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of SERCA1 in mouse C2C12 myoblast cell line - Immunocytochemical staining of fixed and permeabilized C2C12 cells. A. Cells were stained withAnti-SERCA1Antibody (#ACP-011) (1:400)followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells stained in A. C. Merge image of A and B and visualization of cell nuclei using Hoechst 33342 (blue).

product information

CAT :

ACP-011

SKU :

ACP-011-CF_0.2 ml

Product Name :

Anti-SERCA1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64578

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP011

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1

Product Page - Scientific background :

Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.The expression of SERCA1 in mice is essential3 whereas in human its absence is tolerated but is the cause of Brody myopathy4. Malfunction of SERCAs is also observed in heart disease and different forms of cancer1.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1

Short Description :

A Rabbit Polyclonal Antibody to SERCA1 Pump

Long Description :

Anti-SERCA1 Antibody (#ACP-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize SERCA1 from rat, mouse, and human samples.

Negative Control :

BLP-CP011

Positive Control :

Synonyms :

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage