Anti-PMCA1 Antibody | Alomone Labs ACP-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PMCA1 Antibody

catalog :

ACP-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Newton S, Kong F, Carlton A, Aguilar C, Parker A, Codner G, et al. Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. PLoS Genet. 2022;18:e1009937 pubmed publisher
Bächinger D, Egli H, Goosmann M, Monge Naldi A, Eckhard A. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear. Cell Tissue Res. 2019;378:163-173 pubmed publisher
Schmidt N, Kollewe A, Constantin C, Henrich S, Ritzau Jost A, Bildl W, et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron. 2017;96:827-838.e9 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-PMCA1 Antibody (#ACP-005), (1:200).3,4. Anti-PMCA1 Antibody, preincubated with PMCA1 Blocking Peptide (#BLP-CP005).

image 2 :

Expression of PMCA1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA1 Antibody (#ACP-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA1 staining (green) appears in the stratum radiatum (SR) and oriens (OR) layers but not in the pyramidal (Pyr) layer of hippocampal CA1 region (arrows). Nuclei are labeled with DAPI (blue).

image 3 :

Expression of PMCA1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA1Antibody (#ACP-005) (1:300) followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA1 staining (green) appears in the stratum radiatum (SR) and oriens (OR) layers but not in the pyramidal (Pyr) layer of hippocampal CA1 region (arrows). Nuclei are labeled with DAPI (blue).

product information

CAT :

ACP-005

SKU :

ACP-005-CF_0.2 ml

Product Name :

Anti-PMCA1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20020

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP005

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)SGVKNSLKEANHD, corresponding to amino acid residues 12-24 of human PMCA1

Product Page - Scientific background :

The plasma membrane calcium ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells1.PMCA and the sodium/calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations2.Since it transports Ca2+ into the extracellular space, PMCA is also an important regulator of the Ca2+ concentration in the extracellular space3.The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain4.The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, and so is suited to maintaining Ca2+ at its normally very low levels. The NCX is better suited to removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Plasma membrane Ca2+ ATPase 1, ATP2B1

Short Description :

A Rabbit Polyclonal Antibody to PMCA1 ATPase

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Plasma membrane calcium-transporting ATPase 1. Anti-PMCA1 Antibody (#ACP-005) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA1 from mouse, rat, and human samples.

Negative Control :

BLP-CP005

Positive Control :

Synonyms :

Plasma membrane Ca2+ ATPase 1, ATP2B1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2B1

AB Specifictiy :

This antibody will recognize all known PMCA1 splice variants.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage