Anti-PMCA4 Antibody | Alomone Labs ACP-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PMCA4 Antibody

catalog :

ACP-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat stomach lysate (lanes 1 and 3) and rat lung membranes (lanes 2 and 4): - 1,2. Anti-PMCA4 Antibody (#ACP-004), (1:200).3,4. Anti-PMCA4 Antibody, preincubated with PMCA4 Blocking Peptide (#BLP-CP004).

image 2 :

Expression of PMCA4 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA4 Antibody (#ACP-004), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA4 staining (green) appears in neuronal profiles in deep layers of the parietal cortex (arrows). Nuclei are labeled with DAPI (blue).

product information

CAT :

ACP-004

SKU :

ACP-004-CF_0.2 ml

Product Name :

Anti-PMCA4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64542

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP004

Homology :

Mouse - 12/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CDRILNKEGGIVPFKTK, corresponding to amino acid residues 601-617 of rat PMCA4

Product Page - Scientific background :

Plasma membrane calcium pumps (PMCA) are part of the P2 subfamily of P-type primary ion transport ATPases. PMCAs are found in all eukaryotic cells and serve as a major high-affinity transporter for Ca2+ in the plasma membrane.PMCA4, as other PMCA pumps, is predicted to have 10 membrane spanning domains with the -NH2 and -COOH termini both located on the cytosolic side of the membrane. Most of the protein is facing the cytosol and is comprised of three main parts - the intracellular loop between transmembrane segments 2 and 3, the large unit between membrane-spanning domains 4 and 5, and an extended "tail" following the last transmembrane domain.PMCA4 is regulated by various regulatory mechanisms including Ca2+-Calmodulin and acidic phospholipids. Calmodulin binds to a region in the -COOH terminal located about 40 residues downstream from the last transmembrane domain. In the absence of calmodulin, this region acts as an auto-inhibitory domain. Acidic phospholipids are strong activators of PMCA4. They reduce the Michaelis-Menten constant for Ca2+ of the enzyme to roughly 0.3 µM compared with 0.4-0.7 µM in the case of calmodulin activation. Activation by phospholipids makes the PMCA insensitive to calmodulin thus suggesting that their effect is not additive1.PMCA4 deficiency does not affect embryonic development in mice. PMCA4 knockout mice are indistinguishable from wild type mice in terms of their behavior, appearance and weight. PMCA4 is highly expressed in the sperm tail and deletion of PMCA4 causes severely impaired sperm motility in mice2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4

Short Description :

A Rabbit Polyclonal Antibody to PMCA4 ATPase

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat PMCA4. Anti-PMCA4 Antibody (#ACP-004) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA4 from rat and mouse samples. It is unlikely to recognize PMCA4 from human samples.

Negative Control :

BLP-CP004

Positive Control :

Synonyms :

Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2B4

AB Specifictiy :

This antibody is not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage