Anti-PMCA3 Antibody | Alomone Labs ACP-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PMCA3 Antibody

catalog :

ACP-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-PMCA3 Antibody (#ACP-003), (1:200).3,4. Anti-PMCA3 Antibody, preincubated with PMCA3 Blocking Peptide (#BLP-CP003).

image 2 :

Expression of PMCA3 in rat choroid plexus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMCA3 Antibody (#ACP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PMCA3 immunoreactivity (green) appears in the outlines of the choroid plexus (arrows) located in the lateral ventricle of the brain. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACP-003

SKU :

ACP-003-CF_0.2 ml

Product Name :

Anti-PMCA3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64568

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP003

Homology :

Rat, mouse - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFHPKPQ(N)QREVPH, corresponding to amino acid residues 10-23 of rat PMCA3

Product Page - Scientific background :

PMCAs (plasma membrane calcium ATPases) are P-type ATPases, a separate sub-group of Ca2 + pumps responsible for maintain intracellular Ca2+ homeostasis. PMCAs reduce Ca2 + in the cytosol by direct ejection, by controlling the formation of inositol-1,4,5-trisphosphate (IP3), and by decreasing Ca2 + release from the endoplasmic reticulum Ca2+ pool1,2.Four PMAC proteins are expressed in mammals: PMCA1-4. Alternative splicing produces more than twenty variants that are dissimilar in their regulatory characteristics1,3. PMCA pumps have a C-terminus with a high affinity calmodulin-binding site that also acts as an auto-inhibitor. The C-terminal region also has phosphorylation sites as well as proteolytic sites for caspase-3 and calpain and several motifs that control trafficking of the pump1,2.PMCA3 is usually present in excitable cells, in the brain and in hair cells of the inner ear or skeletal muscle. The variant is quickly activated and stays active for a long period of time1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1

Short Description :

A Rabbit Polyclonal Antibody to PMCA3 ATPase

Long Description :

Anti-PMCA3 Antibody (#ACP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA3 from human, rat, and mouse samples.

Negative Control :

BLP-CP003

Positive Control :

Synonyms :

Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2B3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage