Anti-PMCA2 Antibody | Alomone Labs ACP-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PMCA2 Antibody

catalog :

ACP-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Bächinger D, Egli H, Goosmann M, Monge Naldi A, Eckhard A. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear. Cell Tissue Res. 2019;378:163-173 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-PMCA2 Antibody (#ACP-002), (1:400).3,4. Anti-PMCA2 Antibody, preincubated with PMCA2 Blocking Peptide (#BLP-CP002).

image 2 :

Peptide (C)KEIPDPSSINAKTLE corresponding to amino acid residues 522-536 of rat PMCA2 (AccessionP11506). 2ndintracellular loop.

image 3 :

Western blot analysis of rat brain synaptosomal fraction: - 1. Anti-GRIP1 Antibody (#APZ-015) (1:200).2. Anti-GRIP1 Antibody preincubated with the negative control antigen.

product information

CAT :

ACP-002

SKU :

ACP-002-CF_0.2 ml

Product Name :

Anti-PMCA2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11506

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CP002

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2

Product Page - Scientific background :

The plasma membrane calcium ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells1.The PMCA and the sodium/calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations2.Since it transports Ca2+ into the extracellular space, the PMCA is also an important regulator of the Ca2+ concentration in the extracellular space3.The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain4.The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, and so is suited to maintaining Ca2+ at its normally very low levels. The NCX is better suited to removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Plasma membrane Ca2+ ATPase 2, ATP2B2

Short Description :

A Rabbit Polyclonal Antibody to PMCA2 ATPase

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat PMCA2. Anti-PMCA2 Antibody (#ACP-002) can be used in western blot analysis and has been designed to recognize PMCA2 from mouse, rat and human samples.

Negative Control :

BLP-CP002

Positive Control :

Synonyms :

Plasma membrane Ca2+ ATPase 2, ATP2B2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP2B2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage