antibody

Anti-PACC1/TMEM206 (extracellular) Antibody

ACL-031

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ACL-031-CF_0.2 ml

Anti-PACC1/TMEM206 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9D771

IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-CL031

Rat, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular loop.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)KLKHPVMSVSYKEVDR, corresponding to amino acid residues 95 - 110 of mouse PACC1

The proton-activated Cl- (PAC) channel, which opens by extracellular acidic pH, has been well characterized in several cells and tissues by electrophysiology recordings, although its molecular identity was unknown.Recently, two independent groups, identified a two-transmembrane protein named PACC1 (or TMEM206) as the molecular underlie of the proton-activated Cl- channel 1,2.PACC1 has no discernible sequence homology with previously characterized Cl- channels, although its structure of two transmembrane domains with cytoplasmic N- and C-termini and a large extracellular loop, resembles that of the Na+ -selective channels ASICs and ENaCs. In fact, recent work suggests that the functional unit of the proton-activated Cl- channel is a trimeric form of PACC1 subunits3,4.PAC activity that is stimulated by the lowering of extracellular pH and has been recorded in a wide range of mammalian cells and tissues. Acidic pH is crucial for the function of intracellular organelles in the secretory and endocytic pathways and is also one of the pathological hallmarks of many diseases, including cerebral and cardiac ischemia, cancer, infection and inflammation. Therefore, it is no surprise that the involvement of PACC1 in several physiological processes of the endocytic pathway5,6 and pathological conditions such as acid-induced cell death and brain damage after ischemic stroke7 or that upregulation of PACC1 is associated with poor prognosis in patients with hepatocellular carcinoma8.

IFC IHC LCI WB

Alomone Labs

Proton-Activated Chloride Channel, PAC, Transmembrane Protein 206, Proton Activated Chloride Channel 1, Acid-Sensitive Outwardly-Rectifying Anion Channel, ASOR

A Rabbit Polyclonal antibody to PACC1/TMEM206 (extracellular)

Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PACC1 from mouse, rat and human samples.

BLP-CL031

NA

Proton-Activated Chloride Channel, PAC, Transmembrane Protein 206, Proton Activated Chloride Channel 1, Acid-Sensitive Outwardly-Rectifying Anion Channel, ASOR

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

PACC1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:200