Anti-CLIC5 Antibody | Alomone Labs ACL-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLIC5 Antibody

catalog :

ACL-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 3e	Li J, Liu C, Zhao B. N-Terminus of GRXCR2 Interacts With CLIC5 and Is Essential for Auditory Perception. Front Cell Dev Biol. 2021;9:671364 pubmed publisher
	Hatano R, Takeda A, Abe Y, Kawaguchi K, Kazama I, Matsubara M, et al. Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice. Sci Rep. 2018;8:4512 pubmed publisher
	Fan J, Lerner J, Wyatt M, Cai P, Peterson K, Dong L, et al. The klotho-related protein KLPH (lctl) has preferred expression in lens and is essential for expression of clic5 and normal lens suture formation. Exp Eye Res. 2018;169:111-121 pubmed publisher
	Tang T, Lang X, Xu C, Wang X, Gong T, Yang Y, et al. CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nat Commun. 2017;8:202 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 3) and rat lung (lanes 2 and 4) lysates: - 1,2. Anti-CLIC5 Antibody (#ACL-025), (1:200).3,4. Anti-CLIC5 Antibody, preincubated with CLIC5 Blocking Peptide (#BLP-CL025).

image 2 :

Peptide (C)DTNTHGDEKGSQRK corresponding to amino acid residues 161-174 of rat CLIC5 (AccessionQ9EPT8). Intracellular C-terminus.

image 3 :

Western blot analysis of mouse brain lysate: - 1. Anti-GRIP1 Antibody (#APZ-015) (1:200).2. Anti-GRIP1 Antibody preincubated with the negative control antigen.

product information

CAT :

ACL-025

SKU :

ACL-025-CF_0.2 ml

Product Name :

Anti-CLIC5 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9EPT8

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL025

Homology :

Mouse - identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161-174 of rat CLIC5

Product Page - Scientific background :

Chloride (Cl-) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl- channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity; they may reside in either the plasma membrane or the membrane of intracellular compartments or both1. They are detected in the kidney, placenta, intestines, brain and the inner-ear1-4. The vertebrate Cl- intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6); each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel1,5. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl-5.CLIC5, to which there are two isoforms - CLIC5A and CLIC5B - encoded by the same gene5, has first been isolated from human placenta6, and was shown, together with CLIC4 (with which it shares 76% homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton4. CLIC5 shares about 40% homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear2,4. Mice mutated in the Clic5 gene lack coordination and gradually become deaf2, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor7.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Cl- intracellular channel 5

Short Description :

A Rabbit Polyclonal Antibody to CLIC5 Channel

Long Description :

Anti-CLIC5 Antibody (#ACL-025) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize CLIC5 from mouse, rat, and human samples.

Negative Control :

BLP-CL025

Positive Control :

Synonyms :

Cl- intracellular channel 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLIC5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage