Anti-Anoctamin-6 (extracellular) Antibody | Alomone Labs ACL-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Anoctamin-6 (extracellular) Antibody

catalog :

ACL-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4): - 1,2. Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016), (1:200).3,4. Anti-Anoctamin-6 (extracellular) Antibody, preincubated with Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016).

product information

CAT :

ACL-016

SKU :

ACL-016-CF_0.2 ml

Product Name :

Anti-Anoctamin-6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q4KMQ2

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL016

Homology :

Mouse, rat - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6

Product Page - Scientific background :

Anoctamin-6 (ANO6, TMEM16F) is a member of the Anoctamin chloride channel family. This family includes 10 proteins: ANO1-ANO10 that are encoded by the conserved TMEM16A-K genes. Anoctamin-6 acts as calcium (Ca2+)-activated chloride (Cl−) channel. It generates an outwardly rectifying Cl− current when activated by a large increase in local intracellular Ca2+ concentrations1. ANO6 structure includes eight transmembrane domains and cytosolic amino- and carboxyl termini. There is a high sequence identity in the putative transmembrane domains and more divergent in other regions2,3.ANO6 has also been shown to operate as a Ca2+-activated phospholipid scramblase resulting in movement of phosphatidylserine from the inner to the outer leaflet of the plasma membrane bilayer. ANO6 is also involved in bone mineralization, cell volume regulation, cell proliferation and apoptosis1,2. ANO6 is expressed in cyst-forming epithelial cells together with ANO1.A mutation in the ANO6 gene is linked to Scott Syndrome, a rare inherited bleeding disorder, characterized by altered Ca2+-dependent platelet signaling with defective phosphatidylserine exposure and microparticle formation. Deletion of ANO6 in mice shows reduced skeleton size, skeletal deformities, and mineralization defects4,5.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel

Short Description :

A Rabbit Polyclonal Antibody to Anoctamin-6 Channel

Long Description :

Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, and live cell flow cytometry applications. It has been designed to recognize ANO6 from human, rat, and mouse samples.

Negative Control :

BLP-CL016

Positive Control :

Synonyms :

ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ANO6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage