antibody

Anti-Anoctamin-5 Antibody

ACL-015

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

ACL-015-CF_0.2 ml

Anti-Anoctamin-5 Antibody

Antibodies

Antibodies

Polyclonal

Q75UR0

WB

Rat Mouse

Rabbit

BLP-CL015

Rat - identical; human - 12/13 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

2nd intracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5

Anoctamins, also known as TMEM16, is a family of ten proteins involved in a variety of functions including phospholipid scrambling, ion transport and the regulation of other membrane proteins. ANO1 (Anoctamin-1), ANO2 and ANO5 function as Ca2+-activated Cl- channels (CaCCs) and play an important role in calcium dependent chloride secretion. All members of the Anoctamin family present high structural homology and consist of eight transmembrane domains and cytosolic N- and C-termini1.The ANO5 gene is widely distributed and expressed in bone, skeletal muscle, cardiac muscle, brain, lung and kidney and also in the mucosal layer of the GI tract and the skeletal muscle layer of the esophagus of mice2.In phylogenetic analysis ANO5 was found to be a close relative of ANO6. ANO6 has an association with phospholipid scramblase activity. Under both physiological and pathological conditions scramblase dissipates the normal asymmetry of the cell membrane by catalyzing the rapid externalization of phospholipids on the cell membrane and thus promoting apoptosis3.Recessive mutations in the ANO5 gene were found to be linked to limb-girdle muscular dystrophies type 2L (LGMD2L) and non-dysferlin Miyoshi myopathy4. LGMD2L is characterized by proximal weakness, asymmetric quadriceps femoris and atrophy of the biceps brachii muscle5.Mutations in the gene are also linked to the Gnathodiaphyseal dysplasia disease, an unusual generalized autosomal dominant disease. Patients with this syndrome show sclerosis of tubular bones, lesions of jawbones, an increased sensitivity for jaw infection and osteomyeletis and fragile bones6.

WB

Alomone Labs

ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L

A Rabbit Polyclonal Antibody to Anoctamin-5 Channel

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse ANO5. Anti-Anoctamin-5 Antibody (#ACL-015) can be used in western blot analysis. It has been designed to recognize ANO5 from mouse, rat and human samples.

BLP-CL015

NA

ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ANO5

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200