Anti-TMEM16B (ANO2) (extracellular) Antibody | Alomone Labs ACL-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TMEM16B (ANO2) (extracellular) Antibody

catalog :

ACL-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Mura C, Delgado R, Delgado M, Restrepo D, Bacigalupo J. A CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+-activated Cl- current when transfected into HEK293. BMC Neurosci. 2017;18:61 pubmed publisher
Keckeis S, Reichhart N, Roubeix C, Strauß O. Anoctamin2 (TMEM16B) forms the Ca2+-activated Cl- channel in the retinal pigment epithelium. Exp Eye Res. 2017;154:139-150 pubmed publisher
Cherkashin A, Kolesnikova A, Tarasov M, Romanov R, Rogachevskaja O, Bystrova M, et al. Expression of calcium-activated chloride channels Ano1 and Ano2 in mouse taste cells. Pflugers Arch. 2016;468:305-19 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-TMEM16B (ANO2) (extracellular) Antibody (#ACL-012), (1:200).2. Anti-TMEM16B (ANO2) (extracellular) Antibody, preincubated with TMEM16B/ANO2 (extracellular) Blocking Peptide (#BLP-CL012).

image 2 :

Expression of Anoctamin-2 in mouse olfactory bulb - Immunohistochemical staining of mouse olfactory bulb using Anti-TMEM16B (ANO2) (extracellular) Antibody (#ACL-012), (1:200). A. Anoctamin-2 is detected in anoctamin-positive axons (arrows). B. Staining with mouse anti-synaptophysin (red) and DAPI (blue) reveals the glomeruli of the olfactory bulb (asterisk). C. Merge of the above images reveals the spatial relationship of the anotctamin-2 positive axons to the olfactory bulb organization.

image 3 :

Expression of Anoctamin-2 in mouse olfactory bulb - Immunohistochemical staining of mouse olfactory bulb usingAnti-TMEM16B (ANO2) (extracellular) Antibody(#ACL-012) (1:200). A. Anoctamin-2 is detected in anoctamin-positive axons (arrows). B. Staining with mouse anti-synaptophysin (red) and DAPI (blue) reveals the glomeruli of the olfactory bulb (asterisk). C. Merge of the above images reveals the spatial relationship of the anotctamin-2 positive axons to the olfactory bulb organization.

product information

CAT :

ACL-012

SKU :

ACL-012-CF_0.2 ml

Product Name :

Anti-TMEM16B (ANO2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CFW1

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL012

Homology :

Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HSKRPEQWDLDHSLE, corresponding to amino acid residues 632-646 of mouse Anoctamin-2

Product Page - Scientific background :

Anoctamin (ANO or TMEM16) is a family of 10 membrane proteins. This family is named so because these channels are selective to ANions and have eight (OCT) transmembrane domains. Also, these channels are subject to glycosylation in their extracellular loops and have both intracellular N- and C-termini1. Members of this family are expressed in a broad range of different organisms ranging from mammals, flies, worms, plants and yeast1. Alternative splicing is known to affect these channels and regarding their oligomerization state, homedimerization has been observed although when heterologously expressed, these channels may hetero oligomerize2.Ano1 (or TMEM16A, DOG1 and others) the first member to be identified was found to be a Ca2+-activated Cl- channel3-5 therefore other members are likely to also be Cl- channels. These channels are expressed in many different tissues: bronchiolar epithelial cells, pancreatic acinar cells, proximal kidney tubule epithelium, retina, dorsal root ganglia and submandibular gland1. In fact, Ano1 gained a lot of attention as its activation may serve as a therapeutic treatment for cystic fibrosis since it is also expressed in the airways6. These Ca2+-activated Cl- channels are believed to play a role in development as knockout of Ano1 in mice causes abnormal development of the trachea7. Ano2 (TMEM16B) has been shown to mediate Ca2+-activated Cl- current in olfactory epithelium and photoreceptor synapses2,8,9.Although relatively newly discovered channels, they are being discovered in many medical indications. Ano1 has become a marker in gastrointestinal tumors as its expression is significantly upregulated10,11. Similarly, Ano1 is also highly expressed in other carcinomas12,13.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Anoctamin-2, Transmembrane protein 16B, C12orf3

Short Description :

A Rabbit Polyclonal Antibody to Anoctamin-2 (TMEM16B) Channel

Long Description :

Anti-TMEM16B (ANO2) (extracellular) Antibody (#ACL-012) is a highly specific antibody directed against an epitope of mouse Anoctamin-2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Anoctamin-2 from mouse, rat, and human samples.

Negative Control :

BLP-CL012

Positive Control :

Synonyms :

Anoctamin-2, Transmembrane protein 16B, C12orf3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ANO2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage