Anti-TMEM16A (ANO1) (extracellular) Antibody | Alomone Labs ACL-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TMEM16A (ANO1) (extracellular) Antibody

catalog :

ACL-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Zhang Y, Ye L, DUAN D, Yang H, Ma T. TMEM16A Plays an Insignificant Role in Myocardium Remodeling but May Promote Angiogenesis of Heart During Pressure-overload. Front Physiol. 2022;13:897619 pubmed publisher
Kunzelmann K, Centeio R, Wanitchakool P, Cabrita I, Benedetto R, Saha T, et al. Control of Ion Transport by Tmem16a Expressed in Murine Intestine. Front Physiol. 2019;10:1262 pubmed publisher
Rottgen T, Nickerson A, Minor E, Stewart A, Harold A, Rajendran V. Dextran sulfate sodium-induced chronic colitis attenuates Ca2+-activated Cl- secretion in murine colon by downregulating TMEM16A. Am J Physiol Cell Physiol. 2018;315:C10-C20 pubmed publisher
Danielsson J, Vink J, Hyuga S, Fu X, Funayama H, Wapner R, et al. Anoctamin Channels in Human Myometrium: A Novel Target for Tocolysis. Reprod Sci. 2018;25:1589-1600 pubmed publisher
Cherkashin A, Kolesnikova A, Tarasov M, Romanov R, Rogachevskaja O, Bystrova M, et al. Expression of calcium-activated chloride channels Ano1 and Ano2 in mouse taste cells. Pflugers Arch. 2016;468:305-19 pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion (DRG) lysate: - 1. Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011), (1:200).2. Anti-TMEM16A (ANO1) (extracellular) Antibody, preincubated with TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011).

image 2 :

Western blot analysis of rat small intestine lysate: - 1.Anti-TMEM16A (ANO1) (extracellular)Antibody (#ACL-011) (1:200).2. Anti-TMEM16A (ANO1) (extracellular) Antibody preincubated with the control peptide antigen.

image 3 :

Peptide (C)EYVKRKQRYEVDFNLE corresponding to amino acid residues 679-694 of mouse Anoctamin-1 (AccessionQ8BHY3). 3rdextracellular loop.

product information

CAT :

ACL-011

SKU :

ACL-011-CF_0.2 ml

Product Name :

Anti-TMEM16A (ANO1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BHY3

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL011

Homology :

Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EYVKRKQRYEVDFNLE, corresponding to amino acid residues 679-694 of mouse Anoctamin-1

Product Page - Scientific background :

Anoctamin (ANO or TMEM16) is a family of 10 membrane proteins. This family is named so because these channels are selective to ANions and have eight (OCT) transmembrane domains. Also, these channels are subject to glycosylation in their extracellular loops and have both intracellular N- and C-termini1. Members of this family are expressed in a broad range of different organisms ranging from mammals, flies, worms, plants and yeast1. Alternative splicing is known to affect these channels and regarding their oligomerization state, homedimerization has been observed although when heterologously expressed, these channels may hetero oligomerize2.Ano1 (or TMEM16A, DOG1 and others) the first member to be identified was found to be a Ca2+-activated Cl- channel3-5 therefore other members are likely to also be Cl- channels. These channels are expressed in many different tissues: bronchiolar epithelial cells, pancreatic acinar cells, proximal kidney tubule epithelium, retina, dorsal root ganglia and submandibular gland1. In fact, Ano1 gained a lot of attention as its activation may serve as a therapeutic treatment for cystic fibrosis since it is also expressed in the airways6. These Ca2+-activated Cl- channels are believed to play a role in development as knockout of Ano1 in mice causes abnormal development of the trachea7. Ano2 (TMEM16B) has been shown to mediate Ca2+-activated Cl- current in olfactory epithelium and photoreceptor synapses2,8,9.Although relatively newly discovered channels, they are being discovered in many medical indications. Ano1 has become a marker in gastrointestinal tumors as its expression is significantly upregulated10,11. Similarly, Ano1 is also highly expressed in other carcinomas12,13.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Anoctamin-1, Transmembrane protein 16A, DOG-1, ORAOV2, TAOS2

Short Description :

A Rabbit Polyclonal Antibody to Anoctamin-1 (TMEM16A) Channel

Long Description :

Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011) is a highly specific antibody directed against an epitope of mouse Anoctamin-1. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and could potentially detect the protein in living cells. It has been designed to recognize Anoctamin-1 from mouse, rat, and human samples.

Negative Control :

BLP-CL011

Positive Control :

Synonyms :

Anoctamin-1, Transmembrane protein 16A, DOG-1, ORAOV2, TAOS2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ANO1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage