Anti-CLC-7 (CLCN7) Antibody | Alomone Labs ACL-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLC-7 (CLCN7) Antibody

catalog :

ACL-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Duarte Silva E, Meiry da Rocha Araújo S, Oliveira W, Lós D, Bonfanti A, Peron G, et al. Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord. Front Immunol. 2021;12:671511 pubmed publisher

image

image 1 :

Western blot analysis of mouse kidney (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates: - 1,2. Anti-CLC-7 (CLCN7) Antibody (#ACL-008), (1:200).3,4. Anti-CLC-7 (CLCN7) Antibody, preincubated with CLC-7/CLCN7 Blocking Peptide (#BLP-CL008).

product information

CAT :

ACL-008

SKU :

ACL-008-CF_0.2 ml

Product Name :

Anti-CLC-7 (CLCN7) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51799

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL008

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDLARYRLGKGGLE, corresponding to amino acid residues 783-796 of rat CLC-7

Product Page - Scientific background :

Chloride channels (CLCs) are a family of nine anion channels and anion/proton exchangers. While CLC-3 through CLC-7 are anion/proton exchangers, other members of the family seem to function only as anion channels. CLC exchangers, including CLC-7, are mainly expressed in intracellular compartments such as endosomes and lysosomes and are involved in housekeeping regulation of these organelles.The CLC-7 protein is assembled as a dimer with each subunit consisting of 18 transmembrane helices followed by a cytosolic carboxy-terminus that contains two conserved cystathionine-β-synthase (CBS) domains. Three separate chloride binding sites within each subunit were identified in three-dimensional structures, Sint, Scen, and Sext, named after their position relative to the extra- or intra-cellular side of the plasma membrane. The central position forms a significant part of the selectivity filter with the conserved amino acids S107, I109, F357, and Y445 in addition to the less well conserved F348 and I356 coordinating the chloride ion within the conduction pathway1.CLC-7 is vital for the proper function of osteoclast cells. The neutralizing current through CLC-7 is required for an efficient acidification of the cell's resorption lacuna. CLC-7 can be inserted into this specialized plasma membrane domain by an exocytotic insertion from late endosomal-lysosomal membranes. Mice deficient of CLC-7 protein show severe osteoporosis and retinal degeneration2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Chloride channel 7, H+/Cl- exchange transporter 7

Short Description :

A Rabbit Polyclonal Antibody to CLC-7 (CLCN7) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CLC-7. Anti-CLC-7 (CLCN7) Antibody (#ACL-008) can be used in western blot analysis. It has been designed to recognize CLC-7 from human, mouse and rat samples.

Negative Control :

BLP-CL008

Positive Control :

Synonyms :

Chloride channel 7, H+/Cl- exchange transporter 7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLCN7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage