Anti-CFTR Antibody | Alomone Labs ACL-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CFTR Antibody

catalog :

ACL-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 31

Reference
Wuchu F, Ma X, Que Y, Chen J, Ruan Y. Biphasic regulation of CFTR expression by ENaC in epithelial cells: The involvement of Ca2+-modulated cAMP production. Front Cell Dev Biol. 2022;10:781762 pubmed publisher
Hu S, Russell J, Liu S, Cao C, McGaughey J, Rai R, et al. β-Catenin-NF-κB-CFTR interactions in cholangiocytes regulate inflammation and fibrosis during ductular reaction. elife. 2021;10: pubmed publisher
Zhang X, Hothi J, Zhang Y, Ren A, Rock M, Srinivasan S, et al. F1099L-CFTR (c.3297C>G) has Impaired Channel Function and Associates with Mild Disease Phenotypes in Two Pediatric Patients. Life (Basel). 2021;11: pubmed publisher
Liu X, Chen Y, You B, Peng Y, Chen Y, Yang Z, et al. Molecular mechanism mediating enteric bacterial translocation after severe burn: the role of cystic fibrosis transmembrane conductance regulator. Burns Trauma. 2020;8:tkaa042 pubmed publisher
Yin X, Riva L, Pu Y, Martin Sancho L, Kanamune J, Yamamoto Y, et al. MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells. Cell Rep. 2021;34:108628 pubmed publisher
Di Fulvio M, Bogdani M, Velasco M, McMillen T, Ridaura C, Kelly L, et al. Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet. PLoS ONE. 2020;15:e0242749 pubmed publisher
Cabrita I, Kraus A, Scholz J, Skoczynski K, Schreiber R, Kunzelmann K, et al. Cyst growth in ADPKD is prevented by pharmacological and genetic inhibition of TMEM16A in vivo. Nat Commun. 2020;11:4320 pubmed publisher
Berg P, Svendsen S, Sorensen M, Larsen C, Andersen J, Jensen Fangel S, et al. Impaired Renal HCO3- Excretion in Cystic Fibrosis. J Am Soc Nephrol. 2020;31:1711-1727 pubmed publisher
Gr xf3 f I, Bocsik A, Harazin A, Santa Maria A, Vizsnyiczai G, Barna L, et al. The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel. Int J Mol Sci. 2020;21: pubmed publisher
Zhao M, Zhang J, Huang W, Dong J, Guo J, U K, et al. CFTR promotes malignant glioma development via up-regulation of Akt/Bcl2-mediated anti-apoptosis pathway. J Cell Mol Med. 2020;24:7301-7312 pubmed publisher
Centeio R, Cabrita I, Benedetto R, Talbi K, Ousingsawat J, Schreiber R, et al. Pharmacological Inhibition and Activation of the Ca2+ Activated Cl- Channel TMEM16A. Int J Mol Sci. 2020;21: pubmed publisher
Cabrita I, Buchholz B, Schreiber R, Kunzelmann K. TMEM16A drives renal cyst growth by augmenting Ca2+ signaling in M1 cells. J Mol Med (Berl). 2020;98:659-671 pubmed publisher
Liu K, Dong F, Gao H, Guo Y, Li H, Yang F, et al. Promoter hypermethylation of the CFTR gene as a novel diagnostic and prognostic marker of breast cancer. Cell Biol Int. 2020;44:603-609 pubmed publisher
Chiu W, Tran T, Pan S, Huang H, Chen Y, Wong T. Cystic Fibrosis Transmembrane Conductance Regulator: A Possible New Target for Photodynamic Therapy Enhances Wound Healing. Adv Wound Care (New Rochelle). 2019;8:476-486 pubmed publisher
G xe1 l E, Dolen x161 ek J, Sto x17e er A, Pohorec V, xc9 bert A, Venglovecz V. A Novel in situ Approach to Studying Pancreatic Ducts in Mice. Front Physiol. 2019;10:938 pubmed publisher
Xu J, Sun H, Huang G, Liu G, Li Z, Yang H, et al. A fixation method for the optimisation of western blotting. Sci Rep. 2019;9:6649 pubmed publisher
Liu K, Jin H, Guo Y, Liu Y, Wan Y, Zhao P, et al. CFTR interacts with Hsp90 and regulates the phosphorylation of AKT and ERK1/2 in colorectal cancer cells. FEBS Open Bio. 2019;9:1119-1127 pubmed publisher
Phua Y, Chen K, Hemker S, Marrone A, Bodnar A, Liu X, et al. Loss of miR-17~92 results in dysregulation of Cftr in nephron progenitors. Am J Physiol Renal Physiol. 2019;316:F993-F1005 pubmed publisher
Fenton R, Murali S, Kaji I, Akiba Y, Kaunitz J, Kristensen T, et al. Adenylyl Cyclase 6 Expression Is Essential for Cholera Toxin-Induced Diarrhea. J Infect Dis. 2019;220:1719-1728 pubmed publisher
Montoro D, Haber A, Biton M, Vinarsky V, Lin B, Birket S, et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature. 2018;560:319-324 pubmed publisher
Venglovecz V, Pallagi P, Kem ny L, Bal zs A, Balla Z, Becskeh zi E, et al. The Importance of Aquaporin 1 in Pancreatitis and Its Relation to the CFTR Cl- Channel. Front Physiol. 2018;9:854 pubmed publisher
Li D, Wang J, Sun D, Gong X, Jiang H, Shu J, et al. Tanshinone IIA sulfonate protects against cigarette smoke-induced COPD and down-regulation of CFTR in mice. Sci Rep. 2018;8:376 pubmed publisher
Berczeli O, Vizvári E, Katona M, Török D, Szalay L, Rárosi F, et al. Novel Insight Into the Role of CFTR in Lacrimal Gland Duct Function in Mice. Invest Ophthalmol Vis Sci. 2018;59:54-62 pubmed publisher
Huang W, Guo J, Yuan C, Cui Y, Diao F, Yu M, et al. Abnormal CFTR Affects Glucagon Production by Islet ? Cells in Cystic Fibrosis and Polycystic Ovarian Syndrome. Front Physiol. 2017;8:835 pubmed publisher
Honda K, Kim S, Kelly M, Burns J, Constance L, Li X, et al. Molecular architecture underlying fluid absorption by the developing inner ear. elife. 2017;6: pubmed publisher
Huang W, Jin A, Zhang J, Wang C, Tsang L, Cai Z, et al. Upregulation of CFTR in patients with endometriosis and its involvement in NF?B-uPAR dependent cell migration. Oncotarget. 2017;8:66951-66959 pubmed publisher
Zhang J, Wang Y, Chen J, Zhang X, Dong J, Tsang L, et al. Defective CFTR leads to aberrant β-catenin activation and kidney fibrosis. Sci Rep. 2017;7:5233 pubmed publisher
Hahn A, Faulhaber J, Srisawang L, Stortz A, Salomon J, Mall M, et al. Cellular distribution and function of ion channels involved in transport processes in rat tracheal epithelium. Physiol Rep. 2017;5: pubmed publisher
Liu Y, Xu J, Xu L, Wu T, Sun Y, Lee Y, et al. Cystic fibrosis transmembrane conductance regulator mediates tenogenic differentiation of tendon-derived stem cells and tendon repair: accelerating tendon injury healing by intervening in its downstream signaling. FASEB J. 2017;31:3800-3815 pubmed publisher
Liu Z, Guo J, Wang Y, Weng Z, Huang B, Yu M, et al. CFTR-Î²-catenin interaction regulates mouse embryonic stem cell differentiation and embryonic development. Cell Death Differ. 2017;24:98-110 pubmed publisher
Liu K, Zhang X, Zhang J, Tsang L, Jiang X, Chan H. Defective CFTR- β-catenin interaction promotes NF-κB nuclear translocation and intestinal inflammation in cystic fibrosis. Oncotarget. 2016;7:64030-64042 pubmed publisher

image

image 1 :

Western blot analysis of rat lung membranes: - 1. Anti-CFTR Antibody (#ACL-006), (1:200).2. Anti-CFTR Antibody, preincubated with CFTR Blocking Peptide (#BLP-CL006).

image 2 :

Expression of CFTR in rat lungs - Immunohistochemical staining of rat lungs sections using Anti-CFTR Antibody (#ACL-006) (left panel). Strong staining of bronchial epithelial cells (red) and lighter staining of alveolar cells (red-brown) is apparent. There is also positive staining of macrophages while smooth muscle and endothelium are negative. Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.

image 3 :

Western blot analysisof rat lung membranes: - 1.Anti-CFTR Antibody (#ACL-006) (1:200).2. Anti-CFTR Antibody preincubated with the control peptide antigen.

product information

CAT :

ACL-006

SKU :

ACL-006-CF_0.2 ml

Product Name :

Anti-CFTR Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P13569

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL006

Homology :

Mouse, rat, pig - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, C-terminal part

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KEETEEEVQDTRL, corresponding to amino acid residues 1468-1480 of human CFTR

Product Page - Scientific background :

The cystic fibrosis transmembrane conductance regulator (CFTR) is the most dominant Cl- channel in several epithelial tissues, especially in lung and colon. Remarkably, CFTR is a member of the ATP-binding cassette (ABC) transporter superfamily that uses ATP hydrolyzation as the driving force for the translocation of a wide variety of substrates including sugars, amino acids, proteins and hydrophobic compounds, across cellular membranes. The CFTR is unique among ABC transporters in that it is a cAMP-regulated Cl- channel. It shares the superfamily topology of 12 transmembrane domains with two nucleotide-binding domains (NBDs) and a regulatory (R) domain in the large third intracytoplasmic loop that is phosphorylated in multiple sites by PKA. Mutations in the CFTR gene cause channel dysfunction in several ways, ranging from complete loss of surface expression to diminished Cl- secretion. Defects in the CFTR gene cause cystic fibrosis (CF), the most common genetic disease among Caucasians, as well as a form of male sterility.Regulation of the CFTR channel is accomplished through the activation of surface receptors that couple to adenyl cyclase, raise cAMP cellular levels and thus activate PKA. This has been demonstrated for the adenosine and ß2 adrenergic receptor and the vasopressin hormone among others.Besides enhanced Cl- conductance, activation of CFTR also leads to the regulation of other ion channels. The best-studied case is its interaction with the epithelial Na+ channels (ENaC), although it can probably regulate other ion channels as well (Kir1.1 for example). The mechanism by which CFTR regulates other ion channels is not clear, but it may involve protein-protein interactions via molecules that interact with its C-terminal PDZ binding motif, such as the NHERF adaptor protein.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, ABCC7, cAMP-dependent Cl- channel

Short Description :

A Rabbit Polyclonal Antibody to Cystic Fibrosis Transmembrane Conductance Regulator

Long Description :

Anti-CFTR Antibody (#ACL-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CFTR from mouse, rat, and human samples.

Negative Control :

BLP-CL006

Positive Control :

Synonyms :

Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, ABCC7, cAMP-dependent Cl- channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CFTR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage