Anti-CLC-1 (CLCN1) Antibody | Alomone Labs ACL-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLC-1 (CLCN1) Antibody

catalog :

ACL-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Peng Y, Lee Y, Fu S, Chien Y, Liao Y, Chen T, et al. FKBP8 Enhances Protein Stability of the CLC-1 Chloride Channel at the Plasma Membrane. Int J Mol Sci. 2018;19: pubmed publisher

image

image 1 :

Western blot analysis of rat skeletal muscle membranes: - 1. Anti-CLC-1 (CLCN1) Antibody (#ACL-005), (1:200).2. Anti-CLC-1 (CLCN1) Antibody, preincubated with CLC-1/CLCN1 Blocking Peptide (#BLP-CL005).

product information

CAT :

ACL-005

SKU :

ACL-005-CF_0.2 ml

Product Name :

Anti-CLC-1 (CLCN1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35524

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL005

Homology :

Mouse - identical; human, goat - 13/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminal part

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CVHRLGRVLRRKLGED, corresponding to amino acid residues 102-117 of rat CLC-1

Product Page - Scientific background :

CLC-1 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2The ClC-1 channel is almost exclusively expressed in skeletal muscle where it has a dominant role in maintaining the membrane potential at rest and is important for repolarization of the skeletal muscle cells.Mutations in the ClC-1 gene result in myotonia congenita, a condition in which an increase in the excitability of skeletal muscle leads to repetitive action potentials, stiffness, and delayed relaxation. The disease can be either autosomal dominant (Thomsen's disease), or recessive (Becker's myotonia).3

Applications may also work in :

Supplier :

Alomone Labs

Target :

Chloride channel 1, ClC1

Short Description :

A Rabbit Polyclonal Antibody to CLC-1 (CLCN1) Channel

Long Description :

Anti-CLC-1 (CLCN1) Antibody (#ACL-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CLC-1 from rat, mouse, and human samples.

Negative Control :

BLP-CL005

Positive Control :

Synonyms :

Chloride channel 1, ClC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLCN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage