Anti-CLC-K Antibody | Alomone Labs ACL-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLC-K Antibody

catalog :

ACL-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Khayyat N, Zaika O, Tomilin V, Pyrshev K, Pochynyuk O. Angiotensin II increases activity of the ClC-K2 Cl- channel in collecting duct intercalated cells by stimulating production of reactive oxygen species. J Biol Chem. 2021;296:100347 pubmed publisher
Gorinski N, Wojciechowski D, Guseva D, Abdel Galil D, Mueller F, Wirth A, et al. DHHC7-mediated palmitoylation of the accessory protein barttin critically regulates the functions of ClC-K chloride channels. J Biol Chem. 2020;295:5970-5983 pubmed publisher
Curry J, Saurette M, Askari M, Pei L, Filla M, Beggs M, et al. Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease. J Clin Invest. 2020;130:1948-1960 pubmed publisher
Nguyen T, Prykhodko O, Hållenius F, Nyman M. Monobutyrin Reduces Liver Cholesterol and Improves Intestinal Barrier Function in Rats Fed High-Fat Diets. Nutrients. 2019;11: pubmed publisher
Centrone M, De Santo M, Nicotera I, Labate C, Ranieri M, Di Mise A, et al. Gi Protein Modulation of the Potassium Channel TASK-2 Mediates Vesicle Osmotic Swelling to Facilitate the Fusion of Aquaporin-2 Water Channel Containing Vesicles. Cells. 2018;7: pubmed publisher
Dionne L, Shim K, Hoshi M, Cheng T, Wang J, Marthiens V, et al. Centrosome amplification disrupts renal development and causes cystogenesis. J Cell Biol. 2018;217:2485-2501 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-CLC-K Antibody (#ACL-004), (1:200).2. Anti-CLC-K Antibody, preincubated with CLC-K Blocking Peptide (#BLP-CL004).

image 2 :

Expression of creatine transporter in rat hippocampus and cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A8 Antibody (#AGT-008) (1:200) followed by goat anti-rabbit-AlexaFluor-488. A. Creatine transporter staining (green) in the hippocampal CA1 region is detected in apical dendrites (arrows). B. Creatine transporter immunoreactivity (green) in cortex is observed in soma and apical dendrites of neurons (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACL-004

SKU :

ACL-004-CF_0.2 ml

Product Name :

Anti-CLC-K Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51802

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL004

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KKAISTLTNPPAPK, corresponding to amino acid residues 674-687 of the rat longer form CLC-K2L

Product Page - Scientific background :

CLC-Ka and CLC-Kb are members of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. The human CLC-Ka and CLC-Kb (known as CLC-K1 and CLC-K2 in the rat) channels are closely related genes that share 94% sequence homology and identical genomic organization.CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2, CLC-Ka and CLC-Kb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that CLC-4 and CLC-5 (and by inference CLC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved,the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-K channels require the presence of the auxiliary b subunit barttin, a 34 kD transmembrane protein, for transport to the plama membrane and regulation of channel permeation and gating.3CLC-K channels are expressed primarily in the kidney from the thin ascending limb to the collecting duct of the nephron, and in the stria vascularis and dark cells of the vestibular organ of the inner ear.The channels are important for renal salt reabsorption and water balance by enabling chloride exit across the basolateral membranes. The importance of the CLC-K channel in renal function is demonstrated by the fact that loss-of-function mutations in CLC-Kb lead to Bartter syndrome type III, an autosomal recessive disorder characterized by severe salt wasting, low blood pressure, hypokalemia and hypercalciuria.4

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

CLC-K1, CLC-K2, CLC-Ka, CLC-Kb, Clcnka, Clcnkb, ClCk, Chloride channel K

Short Description :

A Rabbit Polyclonal Antibody to CLC-K Channel

Long Description :

Anti-CLC-K Antibody (#ACL-004) is an antibody directed against an epitope of rat CLC-K2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CLC-K channel from rat, mouse, and human samples. The antibody recognizes both CLC-K1 and CLC-K2 isoforms.

Negative Control :

BLP-CL004

Positive Control :

Synonyms :

CLC-K1, CLC-K2, CLC-Ka, CLC-Kb, Clcnka, Clcnkb, ClCk, Chloride channel K

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLCNKA

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage