Anti-CLC-5 Antibody | Alomone Labs ACL-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLC-5 Antibody

catalog :

ACL-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Varela López E, del Valle Mondragon L, Castrejón Tellez V, Perez Torres I, Arenas A, Rojas F, et al. Role of the Transient Receptor Potential Vanilloid Type 1 (TRPV1) in the Regulation of Nitric Oxide Release in Wistar Rat Aorta. Oxid Med Cell Longev. 2021;2021:8531975 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney lysate: - 1. Anti-CLC-5 Antibody (#ACL-003), (1:200).2. Anti-CLC-5 Antibody, preincubated with CLC-5 Blocking Peptide (#BLP-CL003).

image 2 :

Western blot analysis of human HepG2 liver hepatocellular carcinoma: - 1. Anti-SLC6A8 Antibody (#AGT-008) (1:200).2. Anti-SLC6A8 Antibody preincubated with the negative control antigen.

product information

CAT :

ACL-003

SKU :

ACL-003-CF_0.2 ml

Product Name :

Anti-CLC-5 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51796

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL003

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CDYENHFNTSKGGEL, corresponding to amino acid residues 401-415 of rat CLCN5

Product Page - Scientific background :

CLC5 is a member of the voltage-dependent Cl- channel family that includes nine known members. Based on sequence similarity, CLC5 is closely linked to the CLC3 and CLC4 subunits. CLC-5 is mainly found in intracellular vesicles, particularly in early endosomes, where it is believed to contribute to their acidification.The channel is mainly expressed in the kidney, although it has also been found in the small intestine, brain and liver.Mutations in the CLC5 gene cause Dent's disease, an X-linked renal disorder.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS

Short Description :

A Rabbit Polyclonal Antibody to CLC-5 (CLCN5) Channel

Long Description :

Anti-CLC-5 Antibody (#ACL-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CLC-5 from human, mouse, and rat samples.

Negative Control :

BLP-CL003

Positive Control :

Synonyms :

CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLCN5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage