Anti-CLC-2 (CLCN2) Antibody | Alomone Labs ACL-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLC-2 (CLCN2) Antibody

catalog :

ACL-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Rehman S, Narayanan K, Nickerson A, COON S, Hoque K, Sandle G, et al. Parallel intermediate conductance K+ and Cl- channel activity mediates electroneutral K+ exit across basolateral membranes in rat distal colon. Am J Physiol Gastrointest Liver Physiol. 2020;319:G142-G150 pubmed publisher
Okamoto Y, Nagasawa Y, Obara Y, Ishii K, Takagi D, Ono K. Molecular identification of HSPA8 as an accessory protein of a hyperpolarization-activated chloride channel from rat pulmonary vein cardiomyocytes. J Biol Chem. 2019;294:16049-16061 pubmed publisher
Jin Y, Ibrahim D, Magness S, Blikslager A. Knockout of ClC-2 reveals critical functions of adherens junctions in colonic homeostasis and tumorigenicity. Am J Physiol Gastrointest Liver Physiol. 2018;315:G966-G979 pubmed publisher
Scholl U, Stölting G, Schewe J, Thiel A, Tan H, Nelson Williams C, et al. CLCN2 chloride channel mutations in familial hyperaldosteronism type II. Nat Genet. 2018;50:349-354 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CLC-2 (CLCN2) Antibody (#ACL-002), (1:200).2. Anti-CLC-2 (CLCN2) Antibody, preincubated with CLC-2/CLCN2 Blocking Peptide (#BLP-CL002).

image 2 :

Western blot analysis of mouse heart (lanes 1 and 4) mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6)lysates: - 1-3. Anti-SLC6A8 Antibody (#AGT-008) (1:200).4-6. Anti-SLC6A8 Antibody preincubated with the negative control antigen.

product information

CAT :

ACL-002

SKU :

ACL-002-CF_0.2 ml

Product Name :

Anti-CLC-2 (CLCN2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35525

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CL002

Homology :

Mouse - identical; rabbit, swine - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical; guinea pig - 15/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RSRHGLPREGTPSDSDDKC, corresponding to amino acid residues 888-906 of rat CLC-2.

Product Page - Scientific background :

CLC-2 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-2 is widely distributed with prominent expression in brain, kidney, lung and the gastrointestinal system. Mutations in the CLC-2 channel gene detected in humans are associated with idiopathic generalized epilepsies while disruption of the ClC-2 gene in mice is associated with testicular and retinal degeneration. 3,4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Chloride channel 2, ClC2

Short Description :

A Rabbit Polyclonal Antibody to CLC-2 (CLCN2) Channel

Long Description :

Anti-CLC-2 (CLCN2) Antibody (#ACL-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CLC-2 channel from rat, mouse, and human samples.

Negative Control :

BLP-CL002

Positive Control :

Synonyms :

Chloride channel 2, ClC2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLCN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage