Anti-Human TRPV1 (extracellular)-FITC Antibody | Alomone Labs ACC-334-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human TRPV1 (extracellular)-FITC Antibody

catalog :

ACC-334-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Varela López E, del Valle Mondragon L, Castrejón Tellez V, Perez Torres I, Arenas A, Rojas F, et al. Role of the Transient Receptor Potential Vanilloid Type 1 (TRPV1) in the Regulation of Nitric Oxide Release in Wistar Rat Aorta. Oxid Med Cell Longev. 2021;2021:8531975 pubmed publisher

image

image 1 :

Cell surface detection of TRPV1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells: Cell surface detection of TRPV1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Human TRPV1 (extracellular)-FITC Antibody (#ACC-334-F), (5µg).

product information

CAT :

ACC-334-F

SKU :

ACC-334-F_15 mcl

Product Name :

Anti-Human TRPV1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8NER1

Applications :

FC LCI

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CC334

Homology :

Not recommended for rat and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, 1st loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RPVDGLPPFKMEKTGD, corresponding to amino acid residues 456 - 471 of human TRPV1

Product Page - Scientific background :

TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids.4 This channel is associated with tissue injury and inflammation.5,6Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1

Short Description :

A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Human TRPV1 (extracellular) Antibody (#ACC-334) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. This antibody is not recommended for rat and mouse samples. Anti-Human TRPV1 (extracellular)-FITC Antibody (#ACC-334-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. This antibody is not recommended for rat and mouse samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-334), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage