Anti-CHERP Antibody | Alomone Labs ACC-330 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CHERP Antibody

catalog :

ACC-330

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse C2C12 muscle myoblast cell line (lanes 3 and 6) lysates: - 1-3. Anti-CHERP Antibody (#ACC-330), (1:1500).4-6. Anti-CHERP Antibody, preincubated with CHERP Blocking Peptide (#BLP-CC330).

image 2 :

Expression of CHERP in mouse brain - Immunohistochemical staining of mouse brain sections using Anti-CHERP Antibody (#ACC-330). A. In mouse hippocampal CA3 region, CHERP immunoreactivity (green) appears in neurons (arrows) of CA3 pyramidal layer (P). B. In parietal cortex, CHERP staining (green) is detected in neurons (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).

product information

CAT :

ACC-330

SKU :

ACC-330-CF_0.2 ml

Product Name :

Anti-CHERP Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CGZ0

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC330

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP

Product Page - Scientific background :

CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)1-3.CHERP acts as a regulator of both major families of endoplasmic reticulum Ca2+ channels, inositol 1,4,5-trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca2+ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity1,2.CHERP co-localizes with IP3 receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca2+ mobilization, cell growth and proliferation2,3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Short Description :

A Rabbit Polyclonal Antibody to CHERP

Long Description :

Anti-CHERP Antibody (#ACC-330) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CHERP from human, rat, and mouse samples.

Negative Control :

BLP-CC330

Positive Control :

Synonyms :

Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHERP

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1500

more info or order :

Alomone Labs product webpage