Anti-CRACR2A (EFCAB4B) Antibody | Alomone Labs ACC-324 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CRACR2A (EFCAB4B) Antibody

catalog :

ACC-324

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human T cell leukemia (Jurkat) (lanes 1 and 3) and rat stomach (lanes 2 and 4) lysates: - 1,2. Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324), (1:400).3,4. Anti-CRACR2A (EFCAB4B) Antibody, preincubated with CRACR2A/EFCAB4B Blocking Peptide (#BLP-CC324).

image 2 :

Western blot analysis of human T cell leukemia (Jurkat) (lanes 1 and 3) and rat stomach (lanes 2 and 4) lysates: - 12.Anti-CRACR2A (EFCAB4B) Antibody(#ACC-324) (1:400).34. Anti-CRACR2A (EFCAB4B) Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)RSQQHRQGRSHHLE corresponding to amino acid residues 233-246 of rat Prostaglandin F2-? receptor (Accession P43118). Intracellular 3rd loop.

product information

CAT :

ACC-324

SKU :

ACC-324-CF_0.2 ml

Product Name :

Anti-CRACR2A (EFCAB4B) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9BSW2

Applications :

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC324

Homology :

Rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A

Product Page - Scientific background :

Calcium is a vital ion for the functioning of many intracellular processes. When Ca2+ stored in the endoplasmic reticulum is depleted, there is an activation of store-operated channels that results in the influx of Ca2+ from outside the cell. In non-excitable cells and especially in immune cells, most of this influx is carried out by calcium release activated calcium channels also known as "CRACs"1.Mediation of signals from the ER to CRACs and activation of the CRAC channel is conducted by two modulators that physically cluster with the CRAC channel known as Orai1 and STIM1. STIM1 is a single transmembrane segment protein that has a long C-terminal cytoplasmic region and an N-terminus which detects ER Ca2+ depletion. Orai1 is a macromolecular complex with linkers of varying lengths between the ER and plasma membranes.Novel findings suggest the existence of a complex comprised of Orai1, STIM1 and additional proteins. One of these proteins is an EF-hand protein named CRACR2A which regulates Orai-STIM interaction. An EF-hand is a structural domain found in Ca2+ binding proteins consisting of two alpha helices linked by a short loop region.CRACR2A interacts with the cytoplasmic regions of both Orai1 and STIM1 thus creating a tertiary complex. Under high concentrations of Ca2+, CRACR2A caused a dissociation of Orai1 and STIM1 and a mutated CRACR2A was found, in one study, to cause elevated Ca2+ levels that caused cell death in T cells2.Further research is required to fully determine the activation and regulation mechanisms of CRACs and this can be performed by better understanding CRACR2A and its binding proteins.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B

Short Description :

A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human CRAC channel regulator 2A. Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324) can be used in western blot analysis. It has been designed to recognize CRAC channel regulator 2A from rat and human samples.

Negative Control :

BLP-CC324

Positive Control :

Synonyms :

CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CRACR2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage