Anti-CALHM3 Antibody | Alomone Labs ACC-323 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CALHM3 Antibody

catalog :

ACC-323

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) tongue lysates: - 1,2. Anti-CALHM3 Antibody (#ACC-323), (1:400).3,4. Anti-CALHM3 Antibody, preincubated with CALHM3 Blocking Peptide (#BLP-CC323).

product information

CAT :

ACC-323

SKU :

ACC-323-CF_0.2 ml

Product Name :

Anti-CALHM3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q86XJ0

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC323

Homology :

Mouse -14/15 amino acid residues identical; rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KAHLRAISSREQVDR, corresponding to amino acid residues 292-306 of human CALHM3

Product Page - Scientific background :

Calcium homeostasis has major roles in different extra and intra cellular processes and is therefore crucial for cell physiology1.The calcium homeostasis modulator 3 (CalHM3 or protein FAM26A) belongs to a family of six calcium modulating genes mapped to chromosomes 10 and 6. All CALHM genes are present in vertebrates but they lack sequence homology to other known genes.The most researched member of this family is CALHM1 (FAM26C). CALHM1 is a monomer comprised of four transmembrane helices with both the amino and carboxyl termini located in the cytoplasm. Interestingly, this structure is shared with connexins, pannexins and innexins.CALHM channel gating is regulated allosterically by both voltage and Ca2+ concentration. Low concentrations of Ca2+ and depolarization cause channel activation. The molecular basis for voltage-dependent gating remains unknown. CALHM3 channels do not have canonical voltage sensors as found in other channels. CALHM channels poorly discriminate cations and anions enabling signaling molecules such as Ca2+ and ATP to permeate to its pore. This suggests a non-discriminatory ion selectivity filter or a wide ion conduction pore. Recent studies have shown that it is the latter and not the former that accounts for weak ion selectivity2.CALHM3 are specifically expressed in taste buds and localized to TRPM5 cells. Changes in calcium homeostasis have been associated with Alzheimer's disease development. CALHM3 polymorphism can modulate the age for disease onset. In addition, several SNPs within the CALHM3 gene were found to be associated with Creutzfeldt-Jakob disease pathophysiology3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Calcium homeostasis modulator 3, FAM26A

Short Description :

A Rabbit Polyclonal Antibody to Calcium Homeostasis Modulator 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human calcium homeostasis modulator 3. Anti-CALHM3 Antibody (#ACC-323) can be used in western blot analysis. It has been designed to recognize CALHM3 from human, mouse and rat samples.

Negative Control :

BLP-CC323

Positive Control :

Synonyms :

Calcium homeostasis modulator 3, FAM26A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CALHM3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage