Anti-MICU1 Antibody | Alomone Labs ACC-322 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MICU1 Antibody

catalog :

ACC-322

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat skeletal muscle (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates: - 1-3. Anti-MICU1 Antibody (#ACC-322), (1:200).4-6. Anti-MICU1 Antibody, preincubated with MICU1 Blocking Peptide (#BLP-CC322).

image 2 :

Peptide (C)EFERHDPVDGRISER corresponding to amino acid residues 313-327 of rat MICU1 (AccessionQ6P6Q9). Intracellular.

image 3 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1.Anti-CPLX1/2 Antibody (#ANR-011) (1:200).2. Anti-CPLX1/2 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-322

SKU :

ACC-322-CF_0.2 ml

Product Name :

Anti-MICU1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6P6Q9

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC322

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1

Product Page - Scientific background :

Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium-red-sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino-terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C-terminus containing two classical EF-hand Ca2+-binding domains2.Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF-hand domains, exerting an inhibitory role on MCU-dependent Ca2+ entry3.RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS

Short Description :

A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)

Long Description :

Anti-MICU1 Antibody (#ACC-322) is a highly specific antibody directed against an epitope of the rat Mitochondrial calcium uptake protein 1 (CBARA1). The antibody can be used in western blot analysis. It has been designed to recognize CBARA1 from rat, mouse, and human samples.

Negative Control :

BLP-CC322

Positive Control :

Synonyms :

Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MICU1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage