Anti-MCUR1 (CCDC90A) Antibody | Alomone Labs ACC-321 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MCUR1 (CCDC90A) Antibody

catalog :

ACC-321

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Korrapati S, Taukulis I, Olszewski R, Pyle M, Gu S, Singh R, et al. Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis. Front Mol Neurosci. 2019;12:316 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates: - 1-3. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321), (1:200).4-6. Anti-MCUR1 (CCDC90A) Antibody, preincubated with MCUR1/CCDC90A Blocking Peptide (#BLP-CC321).

image 2 :

Western blot analysis of mouse brain (lanes 1 and 4) rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates: - 1-3. Anti-MCUR1 (CCDC90A) Antibody(#ACC-321) (1:200).4-6. Anti-MCUR1 (CCDC90A) Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain lysates: - 12. Anti-CPLX1/2 Antibody (#ANR-011) (1:200).34. Anti-CPLX1/2 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-321

SKU :

ACC-321-CF_0.2 ml

Product Name :

Anti-MCUR1 (CCDC90A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9CXD6

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC321

Homology :

Rat - identical; human - no apparent homology

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop, mitochondrial matrix

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse mitochondrial calcium uniporter regulator 1

Product Page - Scientific background :

Ca2+ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca2+ signals and activation of cell death pathways1. Mitochondrial Ca2+ uptake occurs at regions of close apposition with intracellular Ca2+ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca2+ selective ion channel called the uniporter2,3. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore4. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca2+ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca2+ uptake5.MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space5.MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen5.It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca2+ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca2+ uptake and to ensure normal mitochondrial bioenergetics5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A mitochondrial

Short Description :

A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uniporter Regulator 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse mitochondrial calcium uniporter regulator 1. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321) can be used in western blot analysis. It has been designed to recognize MCUR1 from mouse and rat samples.

Negative Control :

BLP-CC321

Positive Control :

Synonyms :

Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A mitochondrial

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MCUR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage