Anti-CaV1.1 (CACNA1S) (extracellular) Antibody | Alomone Labs ACC-314 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV1.1 (CACNA1S) (extracellular) Antibody

catalog :

ACC-314

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Ramil C, Dong M, An P, Lewandowski T, Yu Z, Miller L, et al. Spirohexene-Tetrazine Ligation Enables Bioorthogonal Labeling of Class B G Protein-Coupled Receptors in Live Cells. J Am Chem Soc. 2017;139:13376-13386 pubmed publisher
Linsley J, Hsu I, Groom L, Yarotskyy V, Lavorato M, Horstick E, et al. Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3. Proc Natl Acad Sci U S A. 2017;114:E228-E236 pubmed publisher

image

image 1 :

Western blot analysis of rat skeletal muscle: - 1. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:200).2. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody, preincubated with Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314).

image 2 :

Expression of CaV1.1 in rat skeletal muscle - Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:100), followed by DAB. CACNA1S staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.

image 3 :

Western blot analysis of rat skeletal muscle: - 1.Anti-CaV1.1 (CACNA1S) (extracellular) Antibody(#ACC-314) (1:200).2. Anti-CaV1.1 (CACNA1S) (extracellular) Antibodypreincubated with the negative control antigen.

product information

CAT :

ACC-314

SKU :

ACC-314-CF_0.2 ml

Product Name :

Anti-CaV1.1 (CACNA1S) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q02485

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC314

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop, domain IV

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CGNVDPDESARIS, corresponding to amino acid residues 506-518 of rat CaV1.1

Product Page - Scientific background :

Voltage-gated Ca2+ channels (VGCCs) serve as key transducers coupling changes in cell surface membrane potential with local intracellular Ca2+ pathways. Among the three families of VGCCs, L-type Ca2+ channels (L-VGCCs), include four different isoforms of the α1 pore-forming subunit: CaV1.1, CaV1.2, CaV1.3 and CaV1.41.CaV1.1 (α1S) is the prototypical VGCCs. It was the first member of the CaV family to be cloned2. CaV1.1 is a single polypeptide composed of four relatively conserved repeats (I, II, II and IV) containing six α-helices a piece. The fourth α-helix of each repeat has a regularly spaced sequence of basic residues that is believed to be critical for voltage-sensing. The I-II-linker is the site of interaction with the intracellular β1a subunit3.CaV1.1 is expressed solely in skeletal muscle. It is localized in regions of the T-tubular membrane that are closely opposed to the sarcoplasmic reticulum (the TSR junction). The primary role of CaV1.1 is to serve as the voltage sensor for skeletal muscle-type excitation contraction (EC) coupling4.Mutations in CaV1.1 have been identified as causative for two congenital muscle pathophysiologies: hypokalemic periodic paralysis5 (HypoKPP) and malignant hyperthermia6.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1S, CACH1, CACN1, CACH1B, CACNL1A3

Short Description :

A Rabbit Polyclonal Antibody to CaV1.1 (CACNA1S) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat CaV1.1 channel. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize CaV1.1 from rat, mouse and human samples.

Negative Control :

BLP-CC314

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1S, CACH1, CACN1, CACH1B, CACNL1A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1S

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage