Anti-CaV1.3 (CACNA1D) (extracellular) Antibody | Alomone Labs ACC-311 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV1.3 (CACNA1D) (extracellular) Antibody

catalog :

ACC-311

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

guinea pig, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:400; loading ...; fig s5b	Axelsson A, Mahdi T, Nenonen H, Singh T, Hänzelmann S, Wendt A, et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Nat Commun. 2017;8:15652 pubmed publisher
immunohistochemistry - paraffin section; guinea pig; 1:50; fig 7	Reyes García J, Flores Soto E, Solís Chagoyán H, Sommer B, Díaz Hernández V, García Hernández L, et al. Tumor Necrosis Factor Alpha Inhibits L-Type Ca(2+) Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 1/2 Pathway. Mediators Inflamm. 2016;2016:5972302 pubmed publisher
	Helm M, Dankovich T, Mandad S, Rammner B, Jähne S, Salimi V, et al. A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines. Nat Neurosci. 2021;24:1151-1162 pubmed publisher
	Eckrich S, Hecker D, Sorg K, Blum K, Fischer K, M xfc nkner S, et al. Cochlea-Specific Deletion of Cav1.3 Calcium Channels Arrests Inner Hair Cell Differentiation and Unravels Pitfalls of Conditional Mouse Models. Front Cell Neurosci. 2019;13:225 pubmed publisher
	Fourbon Y, Gueguinou M, Felix R, Constantin B, Uguen A, Fromont G, et al. Ca2+ protein alpha 1D of CaV1.3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity. Sci Rep. 2017;7:14199 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), and rat C6 brain Glioma (lanes 3 and 6) lysates: - 1-3. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:200).4-6. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody, preincubated with Cav1.3/CACNA1D (extracellular) Blocking Peptide (#BLP-CC311).

image 2 :

Expression of CaV1.3 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311). A. CaV1.3 labeling (red) appears in the cell bodies of the DRG. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of CaV1.3 in rat PC12 cells - Cell surface detection of CaV1.3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells usingAnti-CaV1.3 (CACNA1D) (extracellular)Antibody (#ACC-311) (1:50) (red). B. Merge of A with the live view of the cell.

product information

CAT :

ACC-311

SKU :

ACC-311-CF_0.2 ml

Product Name :

Anti-CaV1.3 (CACNA1D) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P27732

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC311

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop, repeat I

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EQLTKETEGGNHS, corresponding to amino acid residues 215-227 of rat CaV1.3

Product Page - Scientific background :

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1D

Short Description :

A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat protein. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311) can be used in western blot and immunohistochemistry and live cell imaging applications. It is specially suited to detect CaV1.3 channel in live cells. It has been designed to recognize CaV1.3 from mouse, rat and human samples.

Negative Control :

BLP-CC311

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1D

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1D

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage