Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody | Alomone Labs ACC-311-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody

catalog :

ACC-311-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Ca V 1.3 by direct flow cytometry in live intact mouse J774 macrophage cell line:

image 2 :

Cell surface detection of Ca V 1.3 by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:

product information

CAT :

ACC-311-F

SKU :

ACC-311-F_50 mcl

Product Name :

Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P27732

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC311

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop, repeat I

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EQLTKETEGGNHS, corresponding to amino acid residues 215-227 of rat CaV1.3

Product Page - Scientific background :

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1D

Short Description :

A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and flow cytometry applications. It has been designed to recognize CaV1.3 from rat, mouse and human samples. Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody (#ACC-311-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1D

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1D

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-311), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage