Anti-CatSper4 (extracellular) Antibody | Alomone Labs ACC-304 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CatSper4 (extracellular) Antibody

catalog :

ACC-304

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat testis lysate: - 1. Anti-CatSper4 (extracellular) Antibody (#ACC-304), (1:1000).2. Anti-CatSper4 (extracellular) Antibody preincubated with CatSper4 (extracellular) Blocking Peptide (#BLP-CC304).

image 2 :

Peptide (C)NSYLGQKHYELFSTID corresponding to amino acid residues 90-105 of mouse CatSper4 (AccessionQ8BVN3). 1stextracellular loop.

image 3 :

Expression of P2Y2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections withAnti-P2Y2 Receptor (extracellular)Antibody (#APR-102) (1:200) followed by goat-anti-rabbit-AlexaFluor-488. P2RY2 immunoreactivity (green) in the hippocampal dentate gyrus appears in blood vessels (horizontal arrows) and in neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACC-304

SKU :

ACC-304-CF_0.2 ml

Product Name :

Anti-CatSper4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BVN3

Applications :

IC IF WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC304

Homology :

Rat - identical; human -15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4

Product Page - Scientific background :

During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2.CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence TxDxW indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH- and voltage-dependent Ca2+-permeable channel 5. CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6.Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

Cation channel sperm-associated protein 4

Short Description :

A Rabbit Polyclonal Antibody to CatSper4 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse CatSper4. Anti-CatSper4 (extracellular) Antibody (#ACC-304) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CatSper4 from rat, mouse and human samples.

Negative Control :

BLP-CC304

Positive Control :

Synonyms :

Cation channel sperm-associated protein 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CATSPER4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage