Anti-CatSper1 (extracellular) Antibody | Alomone Labs ACC-301 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CatSper1 (extracellular) Antibody

catalog :

ACC-301

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis lysates: - 1.2, Anti-CatSper1 (extracellular) Antibody (#ACC-301), (1:200).3.4, Anti-CatSper1 (extracellular) Antibody, preincubated with CatSper1 (extracellular) Blocking Peptide (#BLP-CC301).

image 2 :

Peptide (C)RALFQDSDPKRFQN corresponding to amino acid residues 508-521 of mouse CatSper1 (AccessionQ91ZR5). 3rdextracellular loop.

image 3 :

Western blot analysis of mouse heart lysate (lanes 1 and 5) mouse brain lysate (lanes 2 and 6) rat brain lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8): - 1-4. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) (1:200).5-8. Anti-P2Y2 Receptor (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-301

SKU :

ACC-301-CF_0.2 ml

Product Name :

Anti-CatSper1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q91ZR5

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC301

Homology :

Rat, human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RALFQDSDPKRFQN, corresponding to amino acid residues 508-521 of mouse CatSper1

Product Page - Scientific background :

Capacitation is the final stage of spermatozoon maturation which eventually enables it to bind the zona pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte1-3. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis)3,4. Capacitation is accompanied by drastic morphological and functional alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pHi1,2, which render the sperm chemotaxis-responsive and hyperactive6. Increased flagellar bend, triggered by Ca2+ signaling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust1-3. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm1-7. CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity7, and is chemotactically responsive to progesterone8. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperβ (2 TM domains), CatSperγ and CatSperδ (1 TM domain each)1, 2. While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits1,3.CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels; a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel2. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+)3. Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Cation channel sperm-associated protein 1

Short Description :

A Rabbit Polyclonal Antibody to CatSper1 Channel

Long Description :

Anti-CatSper1 (extracellular) Antibody (#ACC-301) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting CatSper1 in living cells. It has been designed to recognize CatSper1 from mouse, rat and human samples.

Negative Control :

BLP-CC301

Positive Control :

Synonyms :

Cation channel sperm-associated protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CATSPER1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage