Anti-Pannexin 1 Antibody | Alomone Labs ACC-234 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Pannexin 1 Antibody

catalog :

ACC-234

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:250; loading ...; fig 5a	Vila A, Shihabeddin E, Zhang Z, Santhanam A, Ribelayga C, O BRIEN J. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Front Cell Neurosci. 2021;15:667046 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 1a	Rhodes G, Segars K, Lee Y, Hutcheon A, Rich C, Trinkaus Randall V. Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium. Anal Cell Pathol (Amst). 2021;2021:4793338 pubmed publisher
	Daneva Z, Ottolini M, Chen Y, Klimentova E, Kuppusamy M, Shah S, et al. Endothelial pannexin 1-TRPV4 channel signaling lowers pulmonary arterial pressure in mice. elife. 2021;10: pubmed publisher
	Lee N, Yoon C, Wang Q, Moon S, Koo K, Jung H, et al. Focused Ultrasound Stimulates ER Localized Mechanosensitive PANNEXIN-1 to Mediate Intracellular Calcium Release in Invasive Cancer Cells. Front Cell Dev Biol. 2020;8:504 pubmed publisher
	Rafael A, Cairus A, Tizzoni M, Abudara V, Vitureira N. Glial ATP and Large Pore Channels Modulate Synaptic Strength in Response to Chronic Inactivity. Mol Neurobiol. 2020;57:2856-2869 pubmed publisher
	Arkhipov S, Potter D, Geurts A, Pavlov T. Knockout of P2rx7 purinergic receptor attenuates cyst growth in a rat model of ARPKD. Am J Physiol Renal Physiol. 2019;317:F1649-F1655 pubmed publisher
	Lee Y, Kim M, Rhodes G, Sack K, Son S, Rich C, et al. Sustained Ca2+ mobilizations: A quantitative approach to predict their importance in cell-cell communication and wound healing. PLoS ONE. 2019;14:e0213422 pubmed publisher
	Kristiansen S, Skovsted G, Berchtold L, Radziwon Balicka A, Dreisig K, Edvinsson L, et al. Role of pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion. Scand Cardiovasc J. 2018;52:340-343 pubmed publisher
	Tozzi M, Larsen A, Lange S, Giannuzzo A, Andersen M, Novak I. The P2X7 receptor and pannexin-1 are involved in glucose-induced autocrine regulation in ?-cells. Sci Rep. 2018;8:8926 pubmed publisher
	Molica F, Meens M, Dubrot J, Ehrlich A, Roth C, Morel S, et al. Pannexin1 links lymphatic function to lipid metabolism and atherosclerosis. Sci Rep. 2017;7:13706 pubmed publisher
	Corsini S, Tortora M, Rauti R, Nistri A. Nicotine protects rat hypoglossal motoneurons from excitotoxic death via downregulation of connexin 36. Cell Death Dis. 2017;8:e2881 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 5) and rat (lanes 2 and 6) heart lysates and mouse (lanes 3 and 7) and rat (lanes 4 and 8) brain membranes: - 1-4. Anti-Pannexin 1 Antibody (#ACC-234), (1:400).5-8. Anti-Pannexin 1 Antibody, preincubated with Pannexin 1 Blocking Peptide (#BLP-CC234).

image 2 :

Expression of Pannexin 1 in rat cortex - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Pannexin 1 Antibody (#ACC-234), (1:300). PANX1 staining (green) is apparent in cells with neuronal outline (vertical arrows) and apical dendrites (horizontal arrows) in the parietal cingulated cortex region. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of mouse (lanes 1 and 5) and rat (lanes 2 and 6) heart lysates and mouse (lanes 3 and 7) and rat (lanes 4 and 8) brain membranes: - 1-4.Anti-Pannexin 1 Antibody (#ACC-234) (1:400).5-8. Anti-Pannexin 1 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-234

SKU :

ACC-234-CF_0.2 ml

Product Name :

Anti-Pannexin 1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96RD7

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC234

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1

Product Page - Scientific background :

Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9. Pannexin-1 was shown to interact with NMDA receptors in epilepsy, ischemia, pain and other pathologies10.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PANX1, PX1, MRS1, UNQ2529

Short Description :

A Rabbit Polyclonal Antibody to Pannexin 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Pannexin 1. Anti-Pannexin 1 Antibody (#ACC-234) can be used in western blot and immunohistochemistry applications. It was designed to recognize PANX1 from rat, mouse and human samples.

Negative Control :

BLP-CC234

Positive Control :

Synonyms :

PANX1, PX1, MRS1, UNQ2529

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PANX1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage