Anti-Pannexin 3 Antibody | Alomone Labs ACC-233 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Pannexin 3 Antibody

catalog :

ACC-233

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Buonarati O, Henderson P, Murphy G, Horne M, Hell J. Proteolytic processing of the L-type Ca 2+ channel alpha 11.2 subunit in neurons. F1000Res. 2017;6:1166 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis lysates: - 1,2. Anti-Pannexin 3 Antibody (#ACC-233), (1:200).3,4. Anti-Pannexin 3 Antibody, preincubated with Pannexin 3 Blocking Peptide (#BLP-CC233).

image 2 :

Western blot analysis of rat skeletal muscle lysate: - 1.Anti-Pannexin 3 Antibody (#ACC-233) (1:200).2. Anti-Pannexin 3 Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis lysates: - 12.Anti-Pannexin 3 Antibody (#ACC-233) (1:200).34. Anti-Pannexin 3 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-233

SKU :

ACC-233-CF_0.2 ml

Product Name :

Anti-Pannexin 3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96QZ0

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC233

Homology :

Rat - identical; mouse - 13/15 amino acid residues

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RLVQHMLKIRQKSSD, corresponding to amino acid residues 156-170 of human PANX3

Product Page - Scientific background :

Pannexins are a class of membrane channels similar to the invertebrate Connexin family of gap channel proteins. There are currently three known types of pannexin channels and the exact role of these channels is yet to be fully discerned1.A recent study suggests that despite similarity to the connexin family these channels are not gap-junctions or "hemichannels" as described in some publications but fully operational channels. These conclusions were derived because pannexins, unlike gap-junctions, are found to be expressed and functional in single membranes facing the extracellular space and not as intercellular membranes in appositional cells.Pannexins are comprised of four transmembrane segments, two extracellular loops and both the amino and carboxy termini are facing the cytosol. Pannexins also have four conserved extracellular loop cysteines resulting in the possibility of two intra-pannexin disulfide bonds. Pannexin 3 has a glycosylation site at N254 and N71. Pannexin membrane channels are formed by 6-8 monomers and while pannexins 3's exact oligomeric number has not been determined yet it is expected to be 6 with a greater similarity to Pannexin 1 than 22.There are two known subtypes of Panx3 in mice with different molecular weight and distribution. The first, weighing 43 kDa is mostly expressed in cartilage skin tissues and also in low levels in heart ventricle tissue. The second, weighing 70 kDa, and possibly functioning as a dimer is detected in lung, liver, thymus and spleen tissues3.Since full understanding of pannexin 3 structure, expression and role in physiological and pathological tissues is yet to be obtained there is a definite need for further research.

Applications may also work in :

Supplier :

Alomone Labs

Target :

PANX3, PX3

Short Description :

A Rabbit Polyclonal Antibody to Pannexin 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Pannexin 3. Anti-Pannexin 3 Antibody (#ACC-233) can be used in western blot analysis. It was designed to recognize PANX3 from rat, mouse and human samples.

Negative Control :

BLP-CC233

Positive Control :

Synonyms :

PANX3, PX3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PANX3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage