Anti-Connexin-29 (extracellular) Antibody | Alomone Labs ACC-223 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-29 (extracellular) Antibody

catalog :

ACC-223

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Connexin-29 (extracellular) Antibody (#ACC-223), (1:200).3,4. Anti-Connexin-29 (extracellular) Antibody, preincubated with Connexin-29 (extracellular) Blocking Peptide (#BLP-CC223).

product information

CAT :

ACC-223

SKU :

ACC-223-CF_0.2 ml

Product Name :

Anti-Connexin-29 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q921C1

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC223

Homology :

Mouse - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GNDENEFI(S)HLGQPG, corresponding to amino acid residues 56-70 of mouse Connexin-29

Product Page - Scientific background :

Gap junction channels play an important role in intercellular communication, important for maintaining cell homeostasis. The connexin (Cx) gene family encodes hexameric hemichannels called connexons. When those connexons are docked head to head they form channels. Their presence between adjacent cells form gap junctions. One gap junction is composed of two hexameric connexons and 12 connexins form a single channel1. Connexins have four transmembrane domains, two extracellular loops and one cytoplasmic loop and cytosolic amino and carboxyl termini2.Connexins are responsible for transporting ions and small molecules between the cytoplasm of two attaching cells and between the cytoplasm and the extracellular space2.21 connexins have been identified in humans and 20 in mice2. Connexin 29 was shown to be expressed in the cochlear tissue of mice and rats and in the oligodendrocytes and myelinating Schwann cells in the normal cerebral cortex3,4.Studies show that in mice, connexin 29 expression increases in the penumbra region following traumatic brain injury. Increased expression is also observed in the astrocytes and microglia localized in the injury border4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CX29, Gap junction γ3 protein, Gap junction ε1 protein, GJC3, GJE1

Short Description :

A Rabbit Polyclonal Antibody to Connexin-29

Long Description :

Anti-Connexin-29 (extracellular) Antibody (#ACC-223) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx29 from rat and mouse samples. The antibody will not recognize human samples.

Negative Control :

BLP-CC223

Positive Control :

Synonyms :

CX29, Gap junction γ3 protein, Gap junction ε1 protein, GJC3, GJE1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJC3

AB Specifictiy :

Will not recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage