Anti-Connexin-47 (GJC2) Antibody | Alomone Labs ACC-222 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-47 (GJC2) Antibody

catalog :

ACC-222

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200). 3-4. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).

image 2 :

Expression of Connexin-47 in rat spinal cord ventral horn region. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed CX-47 immunoreactivity (green) in neuron profiles (arrows). B. Pre-incubation of the antibody with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACC-222

SKU :

ACC-222-CF_0.2 ml

Product Name :

Anti-Connexin-47 (GJC2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80XF7

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC222

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47

Product Page - Scientific background :

Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Short Description :

A Rabbit Polyclonal antibody to Connexin-47 (GJC2)

Long Description :

Anti-Connexin-47 (GJC2) Antibody (#ACC-222) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Connexin-47 from rat, mouse and human samples.

Negative Control :

BLP-CC222

Positive Control :

Synonyms :

CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJC2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage