Anti-Connexin-31 (GJB3) Antibody | Alomone Labs ACC-213 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-31 (GJB3) Antibody

catalog :

ACC-213

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Connexin-31 (GJB3) Antibody (#ACC-213), (1:200).3,4. Anti-Connexin-31 (GJB3) Antibody, preincubated with Connexin-31/GJB3 Blocking Peptide (#BLP-CC213).

product information

CAT :

ACC-213

SKU :

ACC-213-CF_0.2 ml

Product Name :

Anti-Connexin-31 (GJB3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28231

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC213

Homology :

Rat - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EINKLLSEQDGSLK, corresponding to amino acid residues 216-228 of mouse Connexin-31

Product Page - Scientific background :

Connexins (Cxs) are the major components of gap junctions, aqueous pores that connect the cytoplasms of two adjacent cells and allow the transfer of ions and small molecules less than 1 kDa between them. Connexins structure includes four trans-membrane domains M1-M4, two extracellular domains E1-E2 and three cytoplasmic domains. The cytoplasmic domains include cytoplasmic loop and carboxy-terminus which are characteristic of each connexin. The membrane spanning and the extracellular domain are highly conserved1,2.Six connexins proteins forms a connexon, also called hemichannel, which can traffic to the plasma membrane and dock with a hemichannel from an adjacent cell to form an intercellular channel. Paracrine signaling and the propagation of Ca2+ waves are two examples of hemichannels functional roles at the plasma membrane. To date, 19 connexin genes have been found in the mouse genome and 20 in the human genome1,3.Connexin 31 (CX31) is highly expressed in the stratum granulosum in the upper differentiating layer of the epidermis. Expression is also detected in peripheral nerves and the cochlea, testis of adults, in placenta, and in developing hindbrain4,5.Mutations in Connexin 31 are associated with multiple human diseases including the genetic skin disorder erythrokeratodermia variabilis, hearing impairment and peripheral neuropathy4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CX31, Gap junction β3 protein

Short Description :

A Rabbit Polyclonal Antibody to Connexin-31

Long Description :

Anti-Connexin-31 (GJB3) Antibody (#ACC-213) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx31 from rat and mouse samples. It will unlikely recognize the protein from human samples.

Negative Control :

BLP-CC213

Positive Control :

Synonyms :

CX31, Gap junction β3 protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJB3

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage