Anti-Connexin-26 (GJB2) Antibody | Alomone Labs ACC-212 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-26 (GJB2) Antibody

catalog :

ACC-212

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Jagannath A, Varga N, Dallmann R, Rando G, Gosselin P, Ebrahimjee F, et al. Adenosine integrates light and sleep signalling for the regulation of circadian timing in mice. Nat Commun. 2021;12:2113 pubmed publisher
Kocovic D, Limaye P, Colburn L, Singh M, Milosevic M, Tadić J, et al. Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex. Cereb Cortex. 2020;30:1244-1259 pubmed publisher
Liu W, Schrott Fischer A, Glueckert R, Benav H, Rask Andersen H. The Human "Cochlear Battery" - Claudin-11 Barrier and Ion Transport Proteins in the Lateral Wall of the Cochlea. Front Mol Neurosci. 2017;10:239 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes: - 1,2. Anti-Connexin-26 (GJB2) Antibody (#ACC-212), (1:200).3,4. Anti-Connexin-26 (GJB2) Antibody, preincubated with Connexin-26/GJB2 Blocking Peptide (#BLP-CC212).

image 2 :

Peptide (C)HEKKRKFMKGEIK corresponding to amino acid residues 100-112 of rat Connexin-26 (AccessionP21994). Intracellular cytoplasmic loop.

image 3 :

Expression of Connexin-26 in human HepG2 liver hepatocellular carcinoma cells - Immunocytochemical staining of fixed and permeabilized HepG2 liver hepatocellular carcinoma cells. A. Cells were stained withAnti-Connexin-26 (GJB2) Antibody (#ACC-212) (1:400) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.

product information

CAT :

ACC-212

SKU :

ACC-212-CF_0.2 ml

Product Name :

Anti-Connexin-26 (GJB2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21994

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC212

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, cytoplasmic loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26

Product Page - Scientific background :

Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1.Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. Mutations in the gene encoding for Connexin-26 protein are associated with deafness7.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

CX26, Gap junction β2 protein

Short Description :

A Rabbit Polyclonal Antibody to Connexin-26

Long Description :

Anti-Connexin-26 (GJB2) Antibody (#ACC-212) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Cx26 from human, rat, and mouse samples.

Negative Control :

BLP-CC212

Positive Control :

Synonyms :

CX26, Gap junction β2 protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage