Anti-Connexin-32 Antibody | Alomone Labs ACC-211 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-32 Antibody

catalog :

ACC-211

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat liver lysate: - 1. Anti-Connexin-32 Antibody (#ACC-211), (1:200).2. Anti-Connexin-32 Antibody, preincubated with Connexin-32 Blocking Peptide (#BLP-CC211).

image 2 :

Peptide (C)EINKLLSEQDGSLK corresponding to amino acid residues 247-260 of rat Connexin-32 (AccessionP08033). Intracellular C-terminus.

image 3 :

Cell surface detection of Zinc transporter ZIP8 in live intact human Jurkat T-cell leukemia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody(#AZT-008-F) (5 g).

product information

CAT :

ACC-211

SKU :

ACC-211-CF_0.2 ml

Product Name :

Anti-Connexin-32 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08033

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC211

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EINKLLSEQDGSLK, corresponding to amino acid residues 247-260 of rat Connexin-32

Product Page - Scientific background :

Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1.Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. A mutation in the gene encoding Connexin-32 is associated with and is the cause for Charcot-Marie-Tooth disease (CMT), a form of demyelinating disease characterized by muscular weakness, affecting more males than females7.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CX32, Gap junction β1 protein, GJB1, CMTX

Short Description :

A Rabbit Polyclonal Antibody to Connexin-32

Long Description :

Anti-Connexin-32 Antibody (#ACC-211) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx32 from human, rat, and mouse samples.

Negative Control :

BLP-CC211

Positive Control :

Synonyms :

CX32, Gap junction β1 protein, GJB1, CMTX

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage