Anti-Connexin-36-ATTO Fluor-488 Antibody | Alomone Labs ACC-209-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-36-ATTO Fluor-488 Antibody

catalog :

ACC-209-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Corsini S, Tortora M, Rauti R, Nistri A. Nicotine protects rat hypoglossal motoneurons from excitotoxic death via downregulation of connexin 36. Cell Death Dis. 2017;8:e2881 pubmed publisher

image

image 1 :

Expression of Connexin-36 in rat spinal cord - Immunohistochemical staining of Connexin-36 in rat spinal cord frozen sections using Anti-Connexin-36-ATTO Fluor-488 Antibody (#ACC-209-AG). A. Cx36 (green) immunoreactive grains scattered along layer I of the dorsal horn (arrows). B. Nuclei were demonstrated using DAPI (blue) as the counterstain. C. Merged image of panel A and panel B.

image 2 :

Expression of Connexin-36 in rat spinal cord - Immunohistochemical staining of Connexin-36 in rat spinal cord frozen sections using Anti-Connexin-36-ATTO-488Antibody(#ACC-209-AG).A. Cx36 (green) immunoreactive grains scattered along layer I of the dorsal horn (arrows).B. Nuclei were demonstrated using DAPI (blue) as the counterstain.C. Merged image of panel A and panel B.

product information

CAT :

ACC-209-AG

SKU :

ACC-209-AG_50 mcl

Product Name :

Anti-Connexin-36-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O70610

Applications :

IF IHC

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC209

Homology :

Mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36

Product Page - Scientific background :

The structural unit of the gap junctions is the connexon. Connexons are hexameric structures, formed by transcellular channels composed of connexins. Connexins (Cx) share a common structural basis, which is a four-transmembrane spanning protein, a cytoplasmic loop, two extracellular loops (E1-E2), one intracellular amino-(NT domain) and a carboxy-terminal (CT domain) region1.Connexin 36 (Cx36) is predominantly expressed in mammalian neuronal cells2. Cx36 is expressed in a variety of mammalian brain regions, but expression is particularly notable in the inferior olive, cerebellar cortex, olfactory bulbs, several brainstem nuclei, the CA3 subregion of the hippocampus and reticular thalamic nuclei3. Outside of the central nervous system, Cx36 also appears in motoneurons of adult rat spinal cord 4.Studies have shown that Cx36 is involved in some neural functions, including neuronal neurogenesis, neural fertility control and regulation of waking and sleep states, and also Cx36 plays an important role in N-methyl-D-aspartate-mediated neuronal death following neuronal injury5. A number of studies performed in humans and animals have confirmed the role of Cx36 in the generation and maintenance of seizure activity6.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9

Short Description :

A Rabbit Polyclonal Antibody to Connexin-36 Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-Connexin-36 Antibody (#ACC-209) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx36 from human, rat, and mouse samples. Anti-Connexin-36-ATTO Fluor-488 Antibody (#ACC-209-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Connexin-36-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJD2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-209), and immunohistochemical staining (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage