Anti-Connexin-45 (GJC1) Antibody | Alomone Labs ACC-207 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-45 (GJC1) Antibody

catalog :

ACC-207

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Singh M, White J, McKimm E, Milosevic M, Antic S. Mechanisms of Spontaneous Electrical Activity in the Developing Cerebral Cortex-Mouse Subplate Zone. Cereb Cortex. 2019;29:3363-3379 pubmed publisher
Trease A, Capuccino J, Contreras J, Harris A, Sorgen P. Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization. J Mol Cell Cardiol. 2017;111:69-80 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1,2. Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:200).3,4. Anti-Connexin-45 (GJC1) Antibody, preincubated with Connexin-45/GJC1 Blocking Peptide (#BLP-CC207).

image 2 :

Expression of Connexin-45 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:400), followed by anti-rabbit-Cy3 antibody. Cx45 staining (red) appears in the neuropil of layers stratum oriens and stratum radiatum but not in the pyramidal layer. Nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: - 1.Anti-Connexin-45 (GJC1) Antibody (#ACC-207) (1:200).2. Anti-Connexin-45 (GJC1) Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-207

SKU :

ACC-207-CF_0.2 ml

Product Name :

Anti-Connexin-45 (GJC1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

A4GG66

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC207

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45

Product Page - Scientific background :

Gap junctions are plasma membrane domains of intercellular channels that allow direct connection between the cytoplasm of adjacent cells. They allow exchange of low molecular weight metabolites, ions and small hydrophilic molecules. These channels are formed by head-to-head docking of hexameric hemichannels called connexons, whose subunit proteins are encoded by the connexin (Cx) gene family1-3.The connexin subunit is formed of four-transmembrane spanning domains, harboring two extracellular loops, one cytoplasmic loop and cytoplasmic N- and C-terminal regions. To date, the family of connexin comprises 21 genes in the human genome4.Connexin 45 (Cx45), encoded by the GJC1 gene, is expressed in cardiomyocytes and in developing vasculature in both endothelial and smooth muscle cells4,5.In the heart, Cx45 along with Cx40 and Cx43, play a crucial role in normal electrical conduction and synchronous heart muscle contraction and important for the normal embryonic development of the heart6. Indeed Cx45 forms low conductance and voltage-sensitive gap junctions between the ventricular cardiac myocytes and the core conduction system2. Studies have showed that cardiac connexin Cx45 is upregulated in human failing hearts and has an important role in the regulation of endothelial-induced mural cell differentiation4,5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7

Short Description :

A Rabbit Polyclonal Antibody to Connexin-45

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-45. Anti-Connexin-45 (GJC1) Antibody (#ACC-207) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx45 from human, rat and mouse samples.

Negative Control :

BLP-CC207

Positive Control :

Synonyms :

CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJC1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage