Anti-Connexin-40 (GJA5) Antibody | Alomone Labs ACC-205 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-40 (GJA5) Antibody

catalog :

ACC-205

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Malmo V, Kelly A, Garten K, Stolen T, Rolim N, Wisloff U, et al. Aerobic Interval Training Prevents Age-Dependent Vulnerability to Atrial Fibrillation in Rodents. Front Physiol. 2018;9:206 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates: - 1,2. Anti-Connexin-40 (GJA5) Antibody (#ACC-205), (1:200).3,4. Anti-Connexin-40 (GJA5) Antibody, preincubated with Connexin-40/GJA5 Blocking Peptide (#BLP-CC205).

image 2 :

Peptide (C)GHRFPQGYHSDKR corresponding to amino acid residues 328-340 of rat Connexin-40 (AccessionP28234). Intracellular C-terminus.

product information

CAT :

ACC-205

SKU :

ACC-205-CF_0.2 ml

Product Name :

Anti-Connexin-40 (GJA5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28234

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC205

Homology :

Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GHRFPQGYHSDKR, corresponding to amino acid residues 328-340 of rat Connexin-40

Product Page - Scientific background :

Connexins are transmembrane proteins, which oligomerize into hexameres adjusting in hemi-channels (connexons). The docking of connexons between two neighboring cells results in the formation of intercellular channels, which are permeable to ions and small molecules1. These channels, which are also known as gap junctions, are characterized by distinct electrophysiological properties. Various physiological and pathological conditions regulate the opening and closing of the channels2. Several genetic disorders have been identified in which specific connexin genes are mutated3.Connexin 40 (Cx40) gene consists two exons, a small untranslated exon, exon 1, and an uninterrupted coding region located in an exon 24. In human and mouse species, Cx40 is expressed in various tissues and cells, such as choriocarcinoma cell lines, tonsil lymphocytes and cardiomyocytes5. The expression of the Cx40 gene in cardiomyocytes suggested that in the adult heart, Cx40 involved in the propagation of the electrical impulses from the atria to the ventricles. It was supported by the finding that Cx40 knockout mice developed cardiac conduction defects which matched the pattern of expression of the gene6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CX40, Gap junction α5 protein

Short Description :

A Rabbit Polyclonal Antibody to Connexin-40

Long Description :

Anti-Connexin-40 (GJA5) Antibody (#ACC-205) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx40 from mouse, rat, and human samples.

Negative Control :

BLP-CC205

Positive Control :

Synonyms :

CX40, Gap junction α5 protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJA5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage