Anti-Connexin-37 Antibody | Alomone Labs ACC-204 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-37 Antibody

catalog :

ACC-204

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse heart lysate (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and mouse lung lysate (lanes 3 and 6): - 1-3. Anti-Connexin-37 Antibody (#ACC-204), (1:400).4-6. Anti-Connexin-37 Antibody, preincubated with Connexin-37 Blocking Peptide (#BLP-CC204).

product information

CAT :

ACC-204

SKU :

ACC-204-CF_0.2 ml

Product Name :

Anti-Connexin-37 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q03190

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC204

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37

Product Page - Scientific background :

Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified1,2. Connexins do not function as single proteins; six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini1,2.Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CX37, Gap junction α4 protein, GJA4

Short Description :

A Rabbit Polyclonal Antibody to Connexin-37

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-37. Anti-Connexin-37 Antibody (#ACC-204) can be used in western blot analysis. It has been designed to recognize Cx37 from rat, mouse and human samples.

Negative Control :

BLP-CC204

Positive Control :

Synonyms :

CX37, Gap junction α4 protein, GJA4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJA4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage