Anti-Connexin-43 Antibody | Alomone Labs ACC-201 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Connexin-43 Antibody

catalog :

ACC-201

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Hammer K, Mustroph J, Stauber T, Birchmeier W, Wagner S, Maier L. Beneficial effect of voluntary physical exercise in Plakophilin2 transgenic mice. PLoS ONE. 2021;16:e0252649 pubmed publisher
Guillebaud F, Barbot M, Barbouche R, Brezun J, Poirot K, Vasile F, et al. Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake. Cells. 2020;9: pubmed publisher
Ivanova E, Kovacs Oller T, Sagdullaev B. Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina. J Comp Neurol. 2019;527:2675-2693 pubmed publisher
Cooper M, Collyer J, Calkins D. Astrocyte remodeling without gliosis precedes optic nerve Axonopathy. Acta Neuropathol Commun. 2018;6:38 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain membranes: - 1. Anti-Connexin-43 Antibody (#ACC-201), (1:400).2. Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).

image 2 :

Expression of Connexin-43 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Connexin-43 Antibody (#ACC-201), (1:300). Cx43 staining (red) appeared in Bergmann glial fibers (arrows) in the molecular layer (MOL) and in the granule layer (G). Cell nuclei were stained with DAPI (blue).

image 3 :

Peptide (C)HAQPFDFPDDNQNSK corresponding to amino acids residues 331-345 of human Connexin-43 (AccessionP17302). Intracellular C-terminus.

product information

CAT :

ACC-201

SKU :

ACC-201-CF_0.2 ml

Product Name :

Anti-Connexin-43 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P17302

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC201

Homology :

Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43

Product Page - Scientific background :

Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate1.Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities2,5,6.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

Short Description :

A Rabbit Polyclonal Antibody to Connexin-43

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Connexin-43. Anti-Connexin-43 Antibody (#ACC-201) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Cx43 from human, rat and mouse samples.

Negative Control :

BLP-CC201

Positive Control :

Synonyms :

CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage