Guinea pig Anti-Connexin-43 Antibody | Alomone Labs ACC-201-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Connexin-43 Antibody

catalog :

ACC-201-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ivanova E, Kovacs Oller T, Sagdullaev B. Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina. J Comp Neurol. 2019;527:2675-2693 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3), (1:1000) and mouse brain (lanes 2 and 4), (1:200) membranes: - 1,2. Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP). 3,4. Guinea pig Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).

image 2 :

Expression of Connexin-43 in mouse brain - Immunohistochemical staining of frozen mouse cerebellum free floating sections using Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP). CX43 immunoreactivity (red) appears in the Purkinje layer (vertical arrows) and in puncta along dendritic processes (horizontal arrows). Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

ACC-201-GP

SKU :

ACC-201-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Connexin-43 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P17302

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC201

Homology :

Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43

Product Page - Scientific background :

Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate1.Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities2,5,6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

Short Description :

A Guinea pig Polyclonal Antibody to Connexin-43

Long Description :

Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP, formerly AGP-141), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CX43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Connexin-43 Antibody (#ACC-201) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC201

Positive Control :

Synonyms :

CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GJA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:1000

more info or order :

Alomone Labs product webpage