Anti-CALHM2 (extracellular)-FITC Antibody | Alomone Labs ACC-142-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CALHM2 (extracellular)-FITC Antibody

catalog :

ACC-142-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of CALHM2 by direct flow cytometry in live intact mouse BV-2 microglia cell line:

image 2 :

Cell surface detection of CALHM2 by direct flow cytometry in live intact human Raji B-cell lymphoblast cell line:

product information

CAT :

ACC-142-F

SKU :

ACC-142-F_50 mcl

Product Name :

Anti-CALHM2 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VEC4

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC142

Homology :

Rat - 13 out of 14 amino acid residues identical Human - 11 out of 14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, 2nd loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2

Product Page - Scientific background :

Calcium homeostasis modulator protein 2 (CALHM2) is a member of the CALHM channel family, a group of transmembrane proteins that play essential roles in calcium ion transport and homeostasis. The CALHM family consist in humans of six members: CALHM1 to CALHM6.1CALHM2 functions as a calcium-permeable ion channel. It allows calcium ions to pass through the cell membrane in response to specific stimuli, such as changes in membrane potential or extracellular calcium concentration.2,3CALHM2 is composed of 323 amino acids and assembles as an undecamer with each protomer containing a large N-terminal transmembrane (TM) domain with 4 TM helices, an intracellular C-terminal domain, and a small extracellular linker region. 2,3CALHMs permeate ions and ATP in a voltage-dependent manner to modulate neuronal excitability. CALHM2 has been shown to accommodate lipids in its hydrophobic channel in order to regulate channel function.2,3Ion and ATP permeation by CALHM proteins play a role in the physiology of cognition, depression, gustatory signaling, and the pathology of Alzheimer's disease.Specifically, CALHM2 expression was increased in Alzheimer's disease (AD) patients and in an AD model in mice. Knockout of CALHM2 improved Aβ plaque deposition, reduced neuroinflammation, and improved cognitive impairment in an AD mice model.4Moreover, microglial CALHM2 may have a central role in both chronic inflammatory diseases (like AD) and in acute inflammatory reactions and neuroinflammation.4

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B

Short Description :

A Rabbit Polyclonal antibody to CALHM2 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-CALHM2 (extracellular) Antibody (#ACC-142) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CALHM2 from mouse, rat and human samples. Anti-CALHM2 (extracellular)-FITC Antibody (#ACC-142-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CALHM2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-142), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage